Optimized DNA sequence encoding Human Endoplasmic reticulum resident protein ERp16/ERp18 (VAL27 - PHE217) extracellular domain including a C-terminal His tag was expressed in HEK293 cells.
Recombinant Human Placental lactogen (CSH1) is a protein consisting of 197 amino acid residue subunits,due to glycosylation migrates as an approximately as 23kDa band protein on reduced SDS-PAGE.
>95%, as determined by SDS-PAGE and HPLC
The activity was measuered by the ability to induce cell proliferation of Nb2‑11 rat lymphoma cells. The calculated ED50 is < 0.2 ng/ml
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant human Placental lactogen(CSH2) is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Recombinant Human Placental lactogen (CSH1) can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.
Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
The placental hormone,
placental lactogen, is a
member of the growth hormone
family and secreted in
humans by the
Placental lactogen is found
in both the maternal and the
fetal circulation , and
during pregnancy, placental
lactogen in humans may play
an important role in the
regulation of maternal
carbohydrate, lipid, and
human Placental lactogen induces cyp2e1 expression via pi 3-kinase pathway in female human hepatocytes
Drug Metab. Dispos.,
492 - 499.
increased levels of cell-free human Placental lactogen mrna at 28-32 gestational weeks in plasma of pregnant women with placenta previa and invasive placenta
215 - 220.
gestational diabetes mellitus resulting from impaired β-cell compensation in the absence of foxm1, a novel downstream effector of Placental lactogen Diabetes,
143 - 152.
alternatively activated macrophages regulate extracellular levels of the hormone Placental lactogen via receptor-mediated uptake and transcytosis
3028 - 3037.
maternal betamethasone administration reduces binucleate cell number and Placental lactogen in sheep
337 - 347.