Optimized DNA sequence encoding Human Cystatin A- CSTA (ILE 2-PHE98) mature chain including a C-terminal His tag was expressed in HEK293 cells.
Recombinant Human Cystatin A (CSTA) is a protein consisting of 127 amino acid residue subunits,due to glycosylation migrates as an approximately as 17kDa band protein on reduced SDS-PAGE.
>95%, as determined by SDS-PAGE and HPLC
The activity was tested by the ability to inhibit Cathepsin L cleavage of a fluorogenic substrate. The ED50 was measured to be 10nM
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant Cystatin A is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Recombinant Human Cystatin A (CSTA) can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.
Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
human cystatin A is an 11-
kDa protein single chain
protease inhibitor capable
of inhibiting papain and
cathepsins B, H, and L, as
well as the cysteine
protease activity of the
major house dust mite
allergen Der p1 .
Cystatin A is expressed at
very low levels in the liver
,cystatin A does not have a
signal peptide for cellular
secretion and is, therefore,
retained within the cell
instead of being secreted .
Overexpression of cystatin A
has been shown to result in
reduced degradation of the
cell cycle regulatory
protein cyclin B in NIH/3T3
fibroblasts and inhibition
of virus-induced apoptosis
in a teleost cell line
imiquimod suppresses propagation of herpes simplex virus 1 by upregulation of Cystatin A via the adenosine receptor a1 pathway
10338 - 10346.
Cystatin A (stefin a), a cysteine protease inhibitor, is involved in the processing of myocilin
modulation of Cystatin A expression in human airway epithelium related to genotype, smoking, copd, and lung cancer
immunomodulatory peptide from cystatin, a natural cysteine protease inhibitor, against leishmaniasis as a model macrophage disease
biological and prognostic role of acid cysteine proteinase inhibitor (acpi, cystatin a) in non-small-cell lung cancer
J. Clin. Pa