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Human Cystatin F Recombinant

CST7

accession:  O76096
   Size A     10ug   $ 160
   Size B      50ug  $680
   Size C      200ug  $ 1950
Domain  : 
Gene  :  CST5
Catalog no. :  RKO76096

 

Source:

Optimized DNA sequence encoding  Human Cystatin F- CST7 (ARG20 - HIS145 ) mature chain including a C-terminal His tag was expressed in HEK293 cells.


 
Molecular weight:

Recombinant Human Cystatin F (CST7) is a protein consisting of 132 amino acid residue subunits,due to glycosylation migrates as an approximately as 17kDa band protein on reduced SDS-PAGE.


 
Purity:
>95%, as determined by SDS-PAGE and HPLC
 

 
Biological Activity:
The activity was tested by the ability to inhibit Cathepsin L cleavage of a fluorogenic substrate. The ED50 was measured to < 7nM
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant Cystatin 7 is lyophilized  from 0.2 μm filtered PBS solution,  pH7.2 ,  5% Trehalose.

              
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
Recombinant Human Cystatin F (CST7) can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.

Avoid repeated freeze/thaw cycles.

 
Usage:
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

Cystatin F

Cystatin F is a recently identified type II cystatin found in humans and mice that possesses a number of unusual properties. The inhibitor shares <35% sequence identity with other type II cystatins and possesses a unique extension of ∼6 amino acids at its N terminus. Compared with other cystatins, the protein exhibits a distinct specificity profile, binding tightly to cathepsins F, K, L, and V, less tightly to cathepsins S and H, and not inhibiting cathepsins B, C, or X . The cysteine cathepsins that are targeted by cystatin F and other cystatin family members are now known to have a range of biological functions that extend beyond catabolism of proteins that enter the endocytic and phagocytic pathway . Cathepsin L (CatL) is a key lysosomal cysteine protease

Related Publications:
internalization of exogenous Cystatin F supresses cysteine proteases and induces the accumulation of single-chain cathepsin l by multiple mechanisms
J. Biol. Chem., Dec 2011; 286: 42082 - 42090.
structural basis of reduction-dependent activation of human Cystatin F
J. Biol. Chem., Jun 2006; 281: 16570 - 16575.




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Antibodies:
Purified Polyclonal
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product_icon_8.jpg Human Cystatin F Recombinant
 
 
 
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