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Human Cystatin D Recombinant

CST5

accession:  P28325
   Size A     10ug   $ 160
   Size B      50ug  $680
   Size C      200ug  $ 1950
Domain  : 
Gene  :  CST5
Catalog no. :  RKP28325

 

Source:

Optimized DNA sequence encoding  Human Cystatin D-CST5(GLY21 - VAL142 ) mature chain including a C-terminal His tag was expressed in HEK293 cells.


 
Molecular weight:

Recombinant Human Cystatin D (CST5) is a protein consisting of 127 amino acid residue subunits,due to glycosylation migrates as an approximately as 17kDa band protein on reduced SDS-PAGE.


 
Purity:
>95%, as determined by SDS-PAGE and HPLC
 

 
Biological Activity:
The activity was tested by the ability to inhibit papain cleavage of a fluorogenic substrate. The ED50 was measured to < 15nM
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant Cystatin 5 is lyophilized  from 0.2 μm filtered PBS solution,  pH7.2 ,  5% Trehalose.

              
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
Recombinant Human Cystatin D (CST5) can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.

Avoid repeated freeze/thaw cycles.

 
Usage:
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

Cystatin D

Cystatin D is a type 2 cystatin, a secreted inhibitor found in human saliva and tear fluid. Compared with its homologues, cystatin D presents an unusual inhibition profile with a preferential inhibition cathepsin S > cathepsin H > cathepsin L and no inhibition of cathepsin B or pig legumain.

Related Publications:
crystal structure of human Cystatin D, a cysteine peptidase inhibitor with restricted inhibition profile
J. Biol. Chem., May 2005; 280: 18221 - 18228.
structural and functional characterization of two allelic variants of human Cystatin D sharing a characteristic inhibition spectrum against mammalian cysteine proteinases
J. Biol. Chem., Sep 1994; 269: 23156 - 23162.
human Cystatin D. cdna cloning, characterization of the escherichia coli expressed inhibitor, and identification of the native protein in saliva.
J. Biol. Chem., Jul 1993; 268: 15737 - 15744.




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Antibodies:
Purified Polyclonal
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product_icon_8.jpg Human Cystatin D Recombinant
 
 
 
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