Optimized DNA sequence encoding Human Cystatin SA-CST2(TRP21 - ALA141) mature chain including a C-terminal His tag was expressed in HEK293 cells.
Recombinant Human Cystatin SA (CST2) is a protein consisting of 127 amino acid residue subunits,due to glycosylation migrates as an approximately as 17kDa band protein on reduced SDS-PAGE.
>95%, as determined by SDS-PAGE and HPLC
The activity was tested by the ability to inhibit Cathepsin L cleavage of a fluorogenic substrate. The ED50 was measured to be 10nM
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant Cystatin SA is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Recombinant Human Cystatin SA (CST2) can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.
Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
identification of Cystatin SA as a novel inhibitor of acid ceramidase
J. Biol. Chem.,
35624 - 35633.
sequence variations of the cst2 gene related to the polymorphism of salivary cystatin sa
Journal of Dental Research,
835 - 839.
identification of full-sized forms of salivary (type) cystatins (cystatin sn, cystatin sa, cystatin s, and two phosphorylated forms of cystatin s) in human whole saliva and determination of phosphorylation sites of cystatin s
648 - 654.
purification, molecular cloning, and sequencing of salivary cystatin sa- 1
J. Biol. Chem.,
9381 - 9387.