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Human Cystatin SA Recombinant


accession:  P09228
   Size A     10ug   $ 160
   Size B      50ug  $680
   Size C      200ug  $ 1950
Domain  : 
Gene  :  CST2
Catalog no. :  RKP09228



Optimized DNA sequence encoding  Human Cystatin SA-CST2(TRP21 - ALA141) mature chain including a C-terminal His tag was expressed in HEK293 cells.

Molecular weight:

Recombinant Human Cystatin SA (CST2) is a protein consisting of 127 amino acid residue subunits,due to glycosylation migrates as an approximately as 17kDa band protein on reduced SDS-PAGE.

>95%, as determined by SDS-PAGE and HPLC

Biological Activity:
The activity was tested by the ability to inhibit Cathepsin L cleavage of a fluorogenic substrate. The ED50 was measured to be 10nM
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

Recombinant Cystatin SA is lyophilized  from 0.2 μm filtered PBS solution,  pH7.2 ,  5% Trehalose.

A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Recombinant Human Cystatin SA (CST2) can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.

Avoid repeated freeze/thaw cycles.

This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Cystatin SA

Related Publications:
identification of Cystatin SA as a novel inhibitor of acid ceramidase
J. Biol. Chem., Oct 2011; 286: 35624 - 35633.
sequence variations of the cst2 gene related to the polymorphism of salivary cystatin sa
Journal of Dental Research, Apr 1999; 78: 835 - 839.
identification of full-sized forms of salivary (type) cystatins (cystatin sn, cystatin sa, cystatin s, and two phosphorylated forms of cystatin s) in human whole saliva and determination of phosphorylation sites of cystatin s
J. Biochem., Oct 1991; 110: 648 - 654.
purification, molecular cloning, and sequencing of salivary cystatin sa- 1
J. Biol. Chem., Jul 1988; 263: 9381 - 9387.

by class:
Growth factors
CD markers
Viral Antigens
by species:
Purified Polyclonal
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