Optimized DNA sequence encoding extracellular domain of Human CD69 (SER62 - LYS199) including a C-terminal His tag was expressed in HEK293 cells.
Recombinant Human CD69 is a protein consisting of 137 amino acid residue subunits,due to glycosylation migrates as an approximately as 23kDa band protein on reduced SDS-PAGE.
>95%, as determined by SDS-PAGE and HPLC
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant human CD69 is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Recombinant CD69 can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.
Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
CD69 is a type II C-type
lectin involved in
lymphocyte migration and
cytokine secretion. CD69 is
homodimer and a member of
the natural killer receptor
family. Its genes are
located in a conserved
genomic region known as the
natural killer gene cluster
on mouse chromosome 6 and
human chromosome 12.CD69
expression represents one of
the earliest available
indicators of leukocyte
activation and its rapid
induction occurs through
In contrast to other
members of the NK C-type
lectin cluster, CD69 has not
been established to have a
role in NK cell recognition
of target cells. In
studies, CD69 inhibited T
cell egress from the thymus
non-traditional cd4+ cd25–CD69+ regulatory t cells is correlated to leukemia relapse after allogeneic hematopoietic stem cell transplantation
differential effect of CD69 targeting on bystander and antigen-specific t cell proliferation
J. Leukoc. Biol.,
145 - 158.
type ii membrane protein CD69 regulates the formation of resting t-helper memory
7409 - 7414.
CD69 regulates type i ifn-induced tolerogenic signals to mucosal cd4 t cells that attenuate their colitogenic potential
2001 - 2013.
activated CD69+ t cells foster immune privilege by regulating ido expression in tumor-associated macrophages
1117 - 1124.