Optimized DNA sequence encoding mature chain of mouse Transcobalamin II - TCII (GLU19 - TRP430 ) including a C-terminal His tag was expressed in HEK293 cells.
Recombinant mouse Transcobalamin II (TC2)is a protein consisting of 419 amino acid residue subunits,due to glycosylation migrates as an approximately as 47kDa band protein on reduced SDS-PAGE.
>95%, as determined by SDS-PAGE and HPLC
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant mouse Transcobalamin II is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Recombinant Transcobalamin II can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.
Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Transcobalamin II is a 45
kDa non-glycoprotein plasma
transporter of Cbl.
Cobalamin complexed to TC II
is endocytosed by a specific
plasma membrane receptor (TC
II-R).The plasma protein
transcobalamin II (TCII)
binds and delivers cobalamin
(Cbl; vitamin B12) to all
cells, which internalize the
Transcobalamin II (TCII).
plasma transport protein for
vitamin B 12. Its
gene has been localised to
transcobalamin ii receptor polymorphisms are associated with increased risk for neural tube defects
J. Med. Genet.,
677 - 685.
multifocal anaplastic astrocytoma in a patient with hereditary colorectal cancer, Transcobalamin II deficiency, agenesis of the corpus callosum, mental retardation, and inherited pms2 mutation
93 - 97.
influence of cobalamin deficiency compared with that of cobalamin absorption on serum holo-transcobalamin ii
Am J Clin Nutr,
110 - 114.
function and stability of human transcobalamin ii: role of intramolecular disulfide bonds c98-c291 and c147-c187
Am J Physiol Cell Physiol,
C150 - C160.
effects of interferon on transcobalamin ii-receptor expression and antitumor activity of nitrosylcobalamin
J Natl Cancer Inst,
1010 - 1019.