Optimized DNA sequence encoding extracellular domain of mouse Disintegrin and metalloproteinase 15- ADAM15 (ASP208 - THR697) including a C-terminal His tag was expressed in HEK293 cells.
Recombinant mouse ADAM15 (MDC15) is a protein consisting of 497 amino acid residue subunits,due to glycosylation migrates as an approximately as 70kDa band protein on reduced SDS-PAGE.
>95%, as determined by SDS-PAGE and HPLC
The activity was tested by the ability of immobilized recombinant mouse ADAM15 to bind Human PTK6 .
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant mouse ADAM15(MDC-15) is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Recombinant ADAM15 can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.
Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
ADAM15 is unique because of
the presence of an Arginine-
Glycine-Aspartic acid (RGD)
motif in its disintegrin
domain that binds αvβ3 and
ADAM15 is among the several
members in the ADAM family
activity, conferred by zinc-
binding protease active
sites in their
enabling cleavage of cell
MDC-15 (metargidin) (13, 14)
is the only known MDC that
has an RGD sequence in its
MDC-15 is widely expressed
in various tissues and
cells, including human
umbilical vein endothelial
cells and smooth muscle
trif-mediated tlr3 and tlr4 signaling is negatively regulated by ADAM15 J. Immunol.,
2217 - 2228.
ADAM15 deficiency attenuates pulmonary hyperpermeability and acute lung injury in lipopolysaccharide-treated mice
Am J Physiol Lung Cell Mol Physiol,
L135 - L142.
ADAM15 protein amplifies focal adhesion kinase phosphorylation under genotoxic stress conditions
J. Biol. Chem.,
21214 - 21223.
exosome release of ADAM15 and the functional implications of human macrophage-derived ADAM15 exosomes
3084 - 3095.
a multi-pronged gene expression approach identifies ADAM15 (metargidin) as a potential therapeutic target for amd
Invest. Ophthalmol. Vis. Sci.,