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Human Carbonic anhydrase IX Recombinant

CAIX

accession:  Q16790
   Size A     10ug   $ 160
   Size B      50ug  $680
   Size C      200ug  $ 1950
Domain  : 
Gene  :  CAIX
Catalog no. :  RKQ16790

 

Source:

Optimized DNA sequence  encoding extracellular domain of human Carbonic anhydrase IX (GLN38 - ASP414) including a C-terminal His tag was expressed in HEK293 cells.


 
Molecular weight:

Recombinant human CAIX is a protein consisting of 384 amino acid residue subunits,due to glycosylation migrates as an approximately as 54kDa band protein on reduced SDS-PAGE.


 
Purity:
>95%, as determined by SDS-PAGE and HPLC
 
Biological activity:
The activity was tested by its esterase activity , the measured activity is 15 pmole/ug/min
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant Human Carbonic anhydrase IX is lyophilized  from 0.2 μm filtered PBS solution, pH7.2 ,  5% Trehalose.

              
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
Recombinant Carbonic anhydrase IX can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.

Avoid repeated freeze/thaw cycles.

 
Usage:
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

Carbonic anhydrase IX

Carbonic anhydrase IX (CA IX) is a cancer-associated transmembrane enzyme catalyzing the reversible reaction CO2 ⇆ H+ + HCO3− involved in ion transport and pH balance. Its catalytic domain (CA) is exposed at the cell surface and contributes to acidification of the outer microenvironment by producing extracellular protons and accelerating CO2 diffusion

Related Publications:
Carbonic anhydrase IX expression is associated with favorable prognostic factors in small intestinal carcinoma
Journal of Histochemistry & Cytochemistry, Mar 2014; 62: 205 - 210.
immunohistochemical reevaluation of Carbonic anhydrase IX (ca ix) expression in tumors and normal tissues
Am J Clin Pathol, Feb 2014; 141: 219 - 225.
results of a phase ii trial of novel Carbonic anhydrase IX radiotracer 18f-vm4-037 in renal cell carcinoma.
ASCO Meeting Abstracts, Feb 2014; 32: LBA400.
Carbonic anhydrase IX is associated with early pulmonary spreading of primary colorectal carcinoma and tobacco smoking
Eur J Cardiothorac Surg, Dec 2013; 10.1093/ejcts/ezt542.
Carbonic anhydrase IX in the prediction of right ventricular dysfunction in patients with hemodynamically stable acute pulmonary embolism
Clinical and Applied Thrombosis/Hemostasis, Apr 2013; 10.1177/1076029613486540.




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