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Mouse Cathepsin L Recombinant

CTSL

accession:  P06797
   Size A     5ug   $ 160
   Size B      20ug  $480
   Size C      100ug  $ 1950
Domain  : 
Gene  :  CTSL
Catalog no. :  RKP06797

 

Source:

Optimized DNA sequence  encoding mature chain of mouse Cathepsin L (THR18 - VAL333) including a C-terminal His tag was expressed in HEK293 cells.


 
Molecular weight:

Recombinant mouse Cathepsin L is a protein consisting of 322 amino acid residue subunits,due to glycosylation migrates as an approximately as 10kDa (mature chain) and 30kDa (propeptide) band protein on reduced SDS-PAGE.


 
Purity:
>95%, as determined by SDS-PAGE and HPLC
 
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant mouse Cathepsin L is lyophilized  from 0.2 μm filtered PBS solution,  pH7.2 ,  5% Trehalose.

              
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
Recombinant Cathepsin L can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.

Avoid repeated freeze/thaw cycles.

 
Usage:
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

Cathepsin L

Cathepsin L, a papain-like cysteine proteinase, is synthesized as a preproenzyme. The prepeptide is removed in the endoplasmic reticulum and the pro-enzyme undergoes autoactivation in the acidic environment of late endosomes and/or lysosomes. The mature form is stored within lysosomes where it functions as an endopeptidase. In addition to its role in terminal protein degradation inside lysosomes, cathepsin L is implicated in multiple physiological and pathological processes, primarily by virtue of its involvement in pro-enzyme processing and protein maturation. For example, in the thymic cortex, cathepsin L plays a key role in cleaving the CLIP peptide thereby allowing for antigen presentation in the context of major histocompatibility complex class II molecules

Related Publications:
trypanosoma brucei cathepsin-l increases arrhythmogenic sarcoplasmic reticulum-mediated calcium release in rat cardiomyocytes
Cardiovasc Res, Nov 2013; 100: 325 - 335.
human papillomavirus type 16 does not require cathepsin l or b for infection
J. Gen. Virol
cathepsin-l ameliorates cardiac hypertrophy through activation of the autophagy–lysosomal dependent protein processing pathways
JAHA, Apr 2013; 2: e000191.
brca1 loss activates cathepsin l–mediated degradation of 53bp1 in breast cancer cells
J. Cel
ab0174 muscle wasting in htnftg mice; an animal model for rheumatoid arthritis; due to increased cathepsin l expression
Ann Rheum Dis, Jun 2012; 71: 647.




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product_icon_8.jpg Mouse Cathepsin L Recombinant
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