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Mouse Cathepsin B Recombinant

CTSB

accession:  P10605
   Size A     10ug   $ 160
   Size B      50ug  $680
   Size C      200ug  $ 1950
Domain  : 
Gene  :  CTSB
Catalog no. :  RKP10605

 

Source:

Optimized DNA sequence  encoding mature chain of mouse Cathepsin B (HIS18 - PHE339) including a C-terminal His tag was expressed in HEK293 cells.


 
Molecular weight:

Recombinant mouse Cathepsin B is a protein consisting of 329 amino acid residue subunits,due to glycosylation migrates as an approximately as 43kDa (propeptide) band protein on reduced SDS-PAGE.


 
Purity:
>95%, as determined by SDS-PAGE and HPLC
 
Biological activity:
The activity was tested by the ability to cleave a flourogenic substrate , the measured activity is 2 nmole/ug/min
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant mouse Cathepsin B is lyophilized  from 0.2 μm filtered PBS solution,  pH7.2 ,  5% Trehalose.

              
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
Recombinant Cathepsin B can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.

Avoid repeated freeze/thaw cycles.

 
Usage:
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

Cathepsin B

CTSB is a lysosomal cysteine protease that plays an important role in physiological protein turnover and processing.he active enzyme is secreted upon lysosomal exocytosis or extracellular processing by surface activators. Cathepsin B is different from other cathepsins in that it functions as an endopeptidase at neutral pH and can be found outside of lysosomes including in the cytoplasm, at the plasma membrane, and in the extracellular space. Cathepsin B contributes to various biological processes including inflammation, cancer metastasis, apoptosis, and differentiation

Related Publications:
glycogen synthase kinase-3β inhibition augments diabetic endothelial progenitor cell abundance and functionality via Cathepsin B: a novel therapeutic opportunity for arterial repair
Diab
Cathepsin B contributes to autophagy-related 7 (atg7)-induced nod-like receptor 3 (nlrp3)-dependent proinflammatory response and aggravates lipotoxicity in rat insulinoma cell line
J. B
Cathepsin B activity has a crucial role in the developmental competence of bovine cumulus–oocyte complexes exposed to heat shock during in vitro maturation
Reproduction, Aug 2013; 146: 407 - 417.
loss of Cathepsin B and z reduces matrix invadosome formation, endothelial sprouting and cnv area in mice
Invest. Ophthalmol. Vis. Sci., Jun 2013; 54: 326.
photodynamic therapy using a protease-mediated theranostic agent reduces cathepsin-b activity in mouse atheromata in vivo
Arterioscler Thromb




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product_icon_8.jpg Mouse Cathepsin B Recombinant
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