Optimized DNA sequence encoding mature chain of human Cathepsin D -CatD (SER19 - LEU412) including a C-terminal His tag was expressed in HEK293 cells.
Recombinant human Cathepsin D (CTSD) is a protein consisting of 402 amino acid residue subunits,due to glycosylation migrates as an approximately as 50kDa band protein on reduced SDS-PAGE.
>95%, as determined by SDS-PAGE and HPLC
The activity was tested by the ability to cleave a flourogenic substrate , the measured activity is 0.5 nmole/ug/min
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant Human Cathepsin D is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Recombinant Cathepsin D can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.
Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Cathepsin D is a soluble
lysosomal aspartic protease
of the pepsin superfamily,
which is widely distributed
in tissues, including the
brain. This protease, after
being synthesized in the
rough endoplasmic reticulum
as preprocathepsin D,
modification to remove the
signal peptide and is then
transported to prelysosomes.
Functionally, the enzyme has
been involved in a variety
of biological activities,
degradation of intracellular
proteins, activation of some
hormones and growth factors,
brain antigen processing,
and regulation of cell death
human herpesvirus 8 interleukin-6 contributes to primary effusion lymphoma cell viability via suppression of proapoptotic Cathepsin D, a cointeraction partner of vitamin k epoxide reductase complex subunit 1 variant 2
1025 - 1038.
can immunohistochemistry quantification of cathepsin-d be useful in the differential diagnosis between vital and post-mortem wounds in humans?
gx15-070 (obatoclax) induces apoptosis and inhibits Cathepsin D- and l–mediated autophagosomal lysis in antiestrogen-resistant breast cancer cells
Mol. Cancer Ther.,
448 - 459.
role of Cathepsin D in u18666a-induced neuronal cell death: potential implication in niemann-pick type c disease pathogenesis
J. Biol. Chem.,
3136 - 3152.
sex- and tissue-specific expression of "similar to nothepsin" and Cathepsin D in relation to egg yolk formation in gallus gallus
2288 - 2293.