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Human Cathepsin D Recombinant

CTSD

accession:  P25774
   Size A     10ug   $ 160
   Size B      50ug  $680
   Size C      200ug  $ 1950
Domain  : 
Gene  :  CTSD
Catalog no. :  RKP07339

 

Source:

Optimized DNA sequence  encoding mature chain of human Cathepsin D -CatD (SER19 - LEU412) including a C-terminal His tag was expressed in HEK293 cells.


 
Molecular weight:

Recombinant human Cathepsin D (CTSD) is a protein consisting of 402 amino acid residue subunits,due to glycosylation migrates as an approximately as 50kDa band protein on reduced SDS-PAGE.


 
Purity:
>95%, as determined by SDS-PAGE and HPLC
 
Biological activity:
The activity was tested by the ability to cleave a flourogenic substrate , the measured activity is 0.5 nmole/ug/min
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant Human Cathepsin D is lyophilized  from 0.2 μm filtered PBS solution,  pH7.2 ,  5% Trehalose.

              
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
Recombinant Cathepsin D can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.

Avoid repeated freeze/thaw cycles.

 
Usage:
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

Cathepsin D

Cathepsin D is a soluble lysosomal aspartic protease of the pepsin superfamily, which is widely distributed in tissues, including the brain. This protease, after being synthesized in the rough endoplasmic reticulum as preprocathepsin D, undergoes post-translational modification to remove the signal peptide and is then transported to prelysosomes. Functionally, the enzyme has been involved in a variety of biological activities, including metabolic degradation of intracellular proteins, activation of some hormones and growth factors, brain antigen processing, and regulation of cell death mechanisms

Related Publications:
human herpesvirus 8 interleukin-6 contributes to primary effusion lymphoma cell viability via suppression of proapoptotic Cathepsin D, a cointeraction partner of vitamin k epoxide reductase complex subunit 1 variant 2
J. Virol., Jan 2014; 88: 1025 - 1038.
can immunohistochemistry quantification of cathepsin-d be useful in the differential diagnosis between vital and post-mortem wounds in humans?
Med
gx15-070 (obatoclax) induces apoptosis and inhibits Cathepsin D- and l–mediated autophagosomal lysis in antiestrogen-resistant breast cancer cells
Mol. Cancer Ther., Apr 2013; 12: 448 - 459.
role of Cathepsin D in u18666a-induced neuronal cell death: potential implication in niemann-pick type c disease pathogenesis
J. Biol. Chem., Feb 2013; 288: 3136 - 3152.
sex- and tissue-specific expression of "similar to nothepsin" and Cathepsin D in relation to egg yolk formation in gallus gallus
Poult. Sci., Sep 2012; 91: 2288 - 2293.




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Antibodies:
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