Optimized DNA sequence encoding mature chain of human Cathepsin B (ARG18 - ILE339) including a C-terminal His tag was expressed in HEK293 cells.
Recombinant human Cathepsin B is a protein consisting of 329 amino acid residue subunits,due to glycosylation migrates as an approximately as 34kDa (mature chain) and 43kDa (propeptide) band protein on reduced SDS-PAGE.
>95%, as determined by SDS-PAGE and HPLC
The activity was tested by the ability to cleave a flourogenic substrate , the measured activity is 3 nM/ug/min
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant Human Cathepsin B is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Recombinant Cathepsin B can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.
Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
CTSB is a lysosomal cysteine
protease that plays an
important role in
turnover and processing.he
active enzyme is secreted
upon lysosomal exocytosis or
extracellular processing by
Cathepsin B is different
from other cathepsins in
that it functions as an
endopeptidase at neutral pH
and can be found outside of
lysosomes including in the
cytoplasm, at the plasma
membrane, and in the
Cathepsin B contributes to
various biological processes
glycogen synthase kinase-3β inhibition augments diabetic endothelial progenitor cell abundance and functionality via Cathepsin B: a novel therapeutic opportunity for arterial repair
Cathepsin B contributes to autophagy-related 7 (atg7)-induced nod-like receptor 3 (nlrp3)-dependent proinflammatory response and aggravates lipotoxicity in rat insulinoma cell line
Cathepsin B activity has a crucial role in the developmental competence of bovine cumulus–oocyte complexes exposed to heat shock during in vitro maturation
407 - 417.
loss of Cathepsin B and z reduces matrix invadosome formation, endothelial sprouting and cnv area in mice
Invest. Ophthalmol. Vis. Sci.,
photodynamic therapy using a protease-mediated theranostic agent reduces cathepsin-b activity in mouse atheromata in vivo