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Human Cathepsin L Recombinant


accession:  P07711
   Size A     10ug   $ 160
   Size B      50ug  $680
   Size C      200ug  $ 1950
Domain  : 
Gene  :  CTSL
Catalog no. :  RKP07711



Optimized DNA sequence  encoding mature chain of human Cathepsin L (THR18 - VAL333) including a C-terminal His tag was expressed in HEK293 cells.

Molecular weight:

Recombinant human Cathepsin L is a protein consisting of 322 amino acid residue subunits,due to glycosylation migrates as an approximately as 10kDa (mature chain) and 30kDa (propeptide) band protein on reduced SDS-PAGE.

>95%, as determined by SDS-PAGE and HPLC
Biological activity:
The activity was tested by the ability of immobilized human CD74 to bind recombinant human Cathepsin L in a linear ange of 40-200 ng/ml
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

Recombinant Human Cathepsin L is lyophilized  from 0.2 μm filtered PBS solution,  pH7.2 ,  5% Trehalose.

A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Recombinant Cathepsin L can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.

Avoid repeated freeze/thaw cycles.

This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Cathepsin L

Cathepsin L, a papain-like cysteine proteinase, is synthesized as a preproenzyme. The prepeptide is removed in the endoplasmic reticulum and the pro-enzyme undergoes autoactivation in the acidic environment of late endosomes and/or lysosomes. The mature form is stored within lysosomes where it functions as an endopeptidase. In addition to its role in terminal protein degradation inside lysosomes, cathepsin L is implicated in multiple physiological and pathological processes, primarily by virtue of its involvement in pro-enzyme processing and protein maturation. For example, in the thymic cortex, cathepsin L plays a key role in cleaving the CLIP peptide thereby allowing for antigen presentation in the context of major histocompatibility complex class II molecules

Related Publications:
trypanosoma brucei cathepsin-l increases arrhythmogenic sarcoplasmic reticulum-mediated calcium release in rat cardiomyocytes
Cardiovasc Res, Nov 2013; 100: 325 - 335.
human papillomavirus type 16 does not require cathepsin l or b for infection
J. Gen. Virol
cathepsin-l ameliorates cardiac hypertrophy through activation of the autophagy–lysosomal dependent protein processing pathways
JAHA, Apr 2013; 2: e000191.
brca1 loss activates cathepsin l–mediated degradation of 53bp1 in breast cancer cells
J. Cel
ab0174 muscle wasting in htnftg mice; an animal model for rheumatoid arthritis; due to increased cathepsin l expression
Ann Rheum Dis, Jun 2012; 71: 647.

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