Optimized DNA sequence encoding mature chain of human Cathepsin L (THR18 - VAL333) including a C-terminal His tag was expressed in HEK293 cells.
Recombinant human Cathepsin L is a protein consisting of 322 amino acid residue subunits,due to glycosylation migrates as an approximately as 10kDa (mature chain) and 30kDa (propeptide) band protein on reduced SDS-PAGE.
>95%, as determined by SDS-PAGE and HPLC
The activity was tested by the ability of immobilized human CD74 to bind recombinant human Cathepsin L in a linear ange of 40-200 ng/ml
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant Human Cathepsin L is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Recombinant Cathepsin L can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.
Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Cathepsin L, a papain-like
cysteine proteinase, is
synthesized as a
preproenzyme. The prepeptide
is removed in the
endoplasmic reticulum and
the pro-enzyme undergoes
autoactivation in the acidic
environment of late
endosomes and/or lysosomes.
The mature form is stored
within lysosomes where it
functions as an
endopeptidase. In addition
to its role in terminal
protein degradation inside
lysosomes, cathepsin L is
implicated in multiple
primarily by virtue of its
involvement in pro-enzyme
processing and protein
maturation. For example, in
the thymic cortex, cathepsin
L plays a key role in
cleaving the CLIP peptide
thereby allowing for antigen
presentation in the context
of major histocompatibility
complex class II molecules
trypanosoma brucei cathepsin-l increases arrhythmogenic sarcoplasmic reticulum-mediated calcium release in rat cardiomyocytes
325 - 335.
human papillomavirus type 16 does not require cathepsin l or b for infection
J. Gen. Virol
cathepsin-l ameliorates cardiac hypertrophy through activation of the autophagy–lysosomal dependent protein processing pathways
brca1 loss activates cathepsin l–mediated degradation of 53bp1 in breast cancer cells
ab0174 muscle wasting in htnftg mice; an animal model for rheumatoid arthritis; due to increased cathepsin l expression
Ann Rheum Dis,