////Mouse Tissue factor pathway inhibitor Recombinant

Mouse Tissue factor pathway inhibitor Recombinant

Category:

$160.00$1,950.00

SKU: RKO54819 Tags: , , ,

Description

Accession
O54819
Source
Optimized DNA sequence encoding mature chain of mouse Tissue factor pathway inhibitor -TFPI (LEU29 - LYS289) including a C-terminal His tag was expressed in HEK293 cells.
Molecular weight
Recombinant mouse TFPI is a protein consisting of 268 amino acid residue subunits,due to glycosylation migrates as an approximately 40kDa band protein on reduced SDS-PAGE.
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological activity
The activity was tested by the ability to inhibit trypsin cleavage of a fluorogenic peptide substrate Mca-RPKPVE- Nval-WRKDnp-NH2, with a calculated ED50 < 0.5nM
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant mouse Tissue factor pathway inhibitoris lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage
Recombinant Tissue factor pathway inhibitor can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to-70°Cfor twelve months. Avoid repeated freeze/thaw cycles.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Biological Process
Molecular function
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