Optimized DNA sequence encoding extracellular domain of Hepatocyte growth factor receptor ,c-MET(GLU25 - ASN929) including a C-terminal His tag was expressed in HEK293 cells.
Recombinant mouse c-MET is a heteredimer protein consisting of alpha and beta chains and contains 915 amino acid residue subunits,due to glycosylation and cleavage migrates as an approximately 45kDa(alpha chain) and 85kDa(beta chain) bands protein on reduced SDS-PAGE.
>95%, as determined by SDS-PAGE and HPLC
The activity was tested by the ability of immobilized recombinant human HGF to bind recombinant mouse MET (HGFR), the calculated ED50 is 2ug/ml
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant Mouse c-MET is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Recombinant mouse HGF receptor can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.
Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
factor/scatter factor c-met
signaling pathway (HGF/SF-
Met) supports diverse
and branching morphogenesis
. HGF/SF-Met signaling is
also of critical importance
particularly in the invasive
and metastatic stages. The
receptor encoded by the c-
met gene is a disulfide
linked α/β heterodimer that
is generated by proteolytic
processing of a single
polypeptide precursor. It
has been demonstrated that
the activation of the
multiple signal transduction
pathways downstream of c-met
takes place via the
multidocking site, a short
sequence motif near the C
terminus of the β chain
chronic lymphocytic leukemia nurse-like cells express the hepatocyte growth factor receptor (c-MET) and indoleamine 2,3-dioxygenase and display features of immunosuppressive type 2 skewed macrophages
tumor suppressor alterations cooperate to drive aggressive mesotheliomas with enriched cancer stem cells via a p53–mir-34a–c-MET axis
1261 - 1271.
c-MET abnormatities in patients with genitourinary (gu) malignancies and outcomes with c-MET inhibitors.
ASCO Meeting Abstracts,
expression of pdgfr-α, egfr and c-MET in spinal chordoma: a series of 52 patients
623 - 630.
rank- and c-MET-mediated signal network promotes prostate cancer metastatic colonization
Endocr. Relat. Cancer,