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Human c-MET (HGFR) Recombinant, Fc

c-MET

accession:  P08581
   Size A     50ug   $ 160
   Size B      200ug  $480
   Size C      1000ug  $ 1950
Domain  : 
Gene  :  MET
Catalog no. :  RFP08581

 

Source:

Optimized DNA sequence  encoding extracellular domain of Hepatocyte growth factor receptor ,c-MET(GLU25 - THR932) including a an IgG1 Fc tag was expressed in HEK293 cells.


 
Molecular weight:

Recombinant human c-MET is a heteredimer protein consisting of alpha and beta chains ,due to glycosylation migrates as an approximately 45kDa (alpha chain) and 125kDa(beta+Fc chain) bands protein on reduced SDS-PAGE.


 
Purity:
>95%, as determined by SDS-PAGE and HPLC
 
Biological activity:
The activity was tested by the ability of immobilized recombinant human HGF to bind recombinant human cMET (HGFR) within a linear range of 300-1500 pg/ml.
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant Human c-MET is lyophilized  from 0.2 μm filtered PBS solution,  pH7.2 ,  5% Trehalose.

              
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
Recombinant HGF receptor can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.

Avoid repeated freeze/thaw cycles.

 
Usage:
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

c-MET

Hepatocyte growth factor/scatter factor c-met signaling pathway (HGF/SF- Met) supports diverse biological processes including development, proliferation, scattering, and branching morphogenesis . HGF/SF-Met signaling is also of critical importance in tumorigenesis, particularly in the invasive and metastatic stages. The receptor encoded by the c- met gene is a disulfide linked α/β heterodimer that is generated by proteolytic processing of a single polypeptide precursor. It has been demonstrated that the activation of the multiple signal transduction pathways downstream of c-met takes place via the multidocking site, a short sequence motif near the C terminus of the β chain

Related Publications:
chronic lymphocytic leukemia nurse-like cells express the hepatocyte growth factor receptor (c-MET) and indoleamine 2,3-dioxygenase and display features of immunosuppressive type 2 skewed macrophages
Haematologica, Feb 2014; 10.3324/haematol.2013.091405.
tumor suppressor alterations cooperate to drive aggressive mesotheliomas with enriched cancer stem cells via a p53–mir-34a–c-MET axis
Cancer Res., Feb 2014; 74: 1261 - 1271.
c-MET abnormatities in patients with genitourinary (gu) malignancies and outcomes with c-MET inhibitors.
ASCO Meeting Abstracts, Feb 2014; 32: 407.
expression of pdgfr-α, egfr and c-MET in spinal chordoma: a series of 52 patients
Anticancer Res, Feb 2014; 34: 623 - 630.
rank- and c-MET-mediated signal network promotes prostate cancer metastatic colonization
Endocr. Relat. Cancer, Jan 2014; 10.1530/ERC-13-0548.




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by species:
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Antibodies:
Purified Polyclonal
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