Optimized DNA sequence encoding extracellular domain of Hepatocyte growth factor receptor ,c-MET(GLU25 - THR932) including a an IgG1 Fc tag was expressed in HEK293 cells.
Recombinant human c-MET is a heteredimer protein consisting of alpha and beta chains ,due to glycosylation migrates as an approximately 45kDa (alpha chain) and 125kDa(beta+Fc chain) bands protein on reduced SDS-PAGE.
>95%, as determined by SDS-PAGE and HPLC
The activity was tested by the ability of immobilized recombinant human HGF to bind recombinant human cMET (HGFR) within a linear range of 300-1500 pg/ml.
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant Human c-MET is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Recombinant HGF receptor can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.
Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
factor/scatter factor c-met
signaling pathway (HGF/SF-
Met) supports diverse
and branching morphogenesis
. HGF/SF-Met signaling is
also of critical importance
particularly in the invasive
and metastatic stages. The
receptor encoded by the c-
met gene is a disulfide
linked α/β heterodimer that
is generated by proteolytic
processing of a single
polypeptide precursor. It
has been demonstrated that
the activation of the
multiple signal transduction
pathways downstream of c-met
takes place via the
multidocking site, a short
sequence motif near the C
terminus of the β chain
chronic lymphocytic leukemia nurse-like cells express the hepatocyte growth factor receptor (c-MET) and indoleamine 2,3-dioxygenase and display features of immunosuppressive type 2 skewed macrophages
tumor suppressor alterations cooperate to drive aggressive mesotheliomas with enriched cancer stem cells via a p53–mir-34a–c-MET axis
1261 - 1271.
c-MET abnormatities in patients with genitourinary (gu) malignancies and outcomes with c-MET inhibitors.
ASCO Meeting Abstracts,
expression of pdgfr-α, egfr and c-MET in spinal chordoma: a series of 52 patients
623 - 630.
rank- and c-MET-mediated signal network promotes prostate cancer metastatic colonization
Endocr. Relat. Cancer,