Optimized DNA sequence Â encoding peptidase domain of Human Hypoxia inducible factor 1 alpha (ARG575 - ASN826) including an N-terminal His tag was expressed in E.Coli.
Recombinant human HIF-1Î± is a protein consisting of 260 amino acid residue subunits, migrates as an approximately 35kDa protein on reduced SDS-PAGE.
>95%, as determined by SDS-PAGE and HPLC
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/Âµg(1EU/Âµg).
Recombinant Human Hypoxia inducible factor 1 is lyophilized Â from 0.2 Î¼m filtered PBS solution, Â pH7.2 , Â 5% Trehalose.
Â Â Â Â Â Â Â Â
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
RecombinantÂ HIF-1Î±Â can be stored in working aliquots at 2Â° - 8Â° C for one month, or at -20Â°C toÂ -70Â°CÂ for twelve months.
Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Hypoxia inducible factor 1
A key factor involved in the
cell response to reduced
oxygen levels is hypoxia-
factor . HIF is a
heterodimer that consists of
a hypoxic response α subunit
and the constitutively
expressed subunit HIF-1β. In
the presence of oxygen, HIF-
α is bound to the von
suppressor protein. This
interaction causes HIF-α to
become ubiquitylated and
targeted to the proteasome,
where it is degraded. Under
hypoxic conditions, HIF-α
becomes stable and binds
HIF-1β to form the active
HIF transcription factor,
which binds to hypoxia-
response elements activating
the expression of numerous
Stabilization of HIF-α in
cancer cells is believed to
be one of the most important
mechanisms that promotes
metastasis and, thereby,
the role of hypoxia-inducible factor-1α and vascular endothelial growth factor in late-phase preconditioning with xenon, isoflurane and levosimendan in rat cardiomyocytes
Interact CardioVasc Thorac Surg,
tirapazamine sensitizes hepatocellular carcinoma cells to topoisomerase i inhibitors via cooperative modulation of hypoxia-inducible factor-1α
Mol. Cancer Ther.,
transcriptional activation of hypoxia-inducible factor-1 (hif-1) in myeloid cells promotes angiogenesis through vegf and s100a8
the apoptosis repressor with a card domain (arc) gene is a direct hypoxia-inducible factor 1 target gene and promotes survival and proliferation of vhl-deficient renal cancer cells
Mol. Cell. Biol.,
anthrax lethal toxin inhibits translation of hypoxia-inducible factor 1α and causes decreased tolerance to hypoxic stress
J. Biol. Chem.,