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Mouse R-Spondin1 Recombinant

RSPO1

accession:  Q9Z132
   Size A     10ug   $ 160
   Size B      50ug  $480
   Size C      500ug  $ 2750
Domain  : 
Gene  :  RSPO1
Catalog no. :  RKQ9Z132

 

Source:

Optimized DNA sequence  encoding peptidase domain of Mouse R-Spondin1(SER21-GLN265 ) including a C-terminal His tag was expressed in HEK293 cells.


 
Molecular weight:

Recombinant Mouse R-Spondin-1 is a protein consisting of 250 amino acid residue subunits,due to glycosylation migrates as an approximately 45kDa protein on reduced SDS-PAGE.


 
Purity:
>95%, as determined by SDS-PAGE and HPLC
 
Biological Activity:
The activity was tested by the ability recombinant mouse RSPO1 to induce activation of beta catenin in Luciferase assay in the presence of recombinant mouse Wnt3a, the calulated ED50 is 50-200 ng/ml.
 
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant Mouse R-spondin1 is lyophilized  from 0.2 μm filtered PBS solution,  pH7.2 ,  5% Trehalose.

              
Storage:
Recombinant R-spondin1 can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.

Avoid repeated freeze/thaw cycles.

 
Usage:
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

R-Spondin1

The R-Spondin (RSpo) family of secreted proteins is implicated in the amplification of canonical Wnt/β-catenin signaling pathway both in vitro and in vivo. All four RSpo family members contain an N- terminal signal peptide, two furin-like domains, followed by a thrombospondin type1 domain, and a C-terminal region rich with positively charged amino acids. The Wnt/β-catenin signaling plays a pivotal role in both embryonic development and homeostatic self-renewal of adult tissues, including gastrointestinal (GI) and oral mucosa. Human RSPO1 was first identified by its ability to stimulate the growth of intestinal epithelium in transgenic mice. The therapeutic potential of the RSPO1 protein was also demonstrated in mouse models of cancer-therapy-induced mucositis and experimental colitis

Related Publications:
the wnt agonist R-Spondin1 regulates systemic graft-versus-host disease by protecting intestinal stem cells
J. Exp. Med., Feb 2011; 208: 285 - 294.
R-Spondin1 protects mice from chemotherapy or radiation-induced oral mucositis through the canonical wnt/β-catenin pathway
PNAS, Feb 2009; 106: 2331 - 2336.
use of R-Spondin1, an intestinotrophic mitogen, in the treatment of murine graft-versus-host disease
Blood (ASH Annual Meeting Abstracts), Nov 2008; 112: 3520.
protection of the intestinal epithelium from conditioning with R-Spondin1 ameliorates graft-versus-host disease
Blood (ASH Annual Meeting Abstracts), Nov 2008; 112: 66.
crosstalk between ahr and wnt signaling through R-Spondin1 impairs tissue regeneration in zebrafish
FASEB J, Aug 2008; 22: 3087 - 3096.




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Antibodies:
Purified Polyclonal
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