Optimized DNA sequence encoding peptidase domain of Mouse Carboxypeptidase E-CPE (GLN28-PHE476 ) including a C-terminal His tag was expressed in HEK293 cells.
Recombinant mouse NGAL is a protein consisting of 456 amino acid residue subunits,due to glycosylation migrates as an approximately 53kDa protein on reduced SDS-PAGE.
>95%, as determined by SDS-PAGE and HPLC
The activity was tested by the ability to cleave a peptide substrate, benzoylAROH, and was measured to be 10 nMole/min/ug .
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant Mouse Carboxypeptidase E (CPE) is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
Recombinant CPE can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.
Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
CARBOXYPEPTIDASE E (CPE) is
a processing enzyme that is
highly expressed in
endocrine cells and
peptidergic neurons . It
functions to cleave carboxy-
terminally extended lysine
and arginine residues from
peptide hormone and
to form bioactive peptides
in the regulated secretory
pathway (RSP). In addition
to its enzymatic function,
CPE has been shown to
facilitate trafficking of
several prohormones into the
granules of the RSP.
interleukin-17-induced protein lipocalin 2 is dispensable for immunity to oral candidiasis
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