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Human Dickkopf-1 (DKK1) Recombinant


accession:  O94907
   Size A     10ug   $ 160
   Size B      50ug  $480
   Size C      500ug  $ 3500
Domain  : 
Gene  :  DKK1
Catalog no. :  RKO94907



Optimized DNA sequence  encoding  extracellular domain of Human Dickkopf-1 (DKK-1)(THR32 - HIS266) including a C-terminal His tag was expressed in HEK293 cells.

Molecular weight:

Recombinant human DKK1 is a protein consisting of 240 amino acid residue subunits,due to glycosylation migrates as an approximately 40kDa protein on reduced SDS-PAGE.

>95%, as determined by SDS-PAGE and HPLC
Biological Activity:
The activity was tested by the ability to inhibit Wnt3-ainduced alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells.
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

Recombinant Human Dickkopf-1 is lyophilized  from 0.2 μm filtered PBS solution,  pH7.2 ,  5% Trehalose.

Recombinant  DKK1 can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.

Avoid repeated freeze/thaw cycles.

This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.



The Dickkopf (Dkk) protein family, which comprises four members (Dkk1 to Dkk4), binds to LRP6 and antagonizes the β-catenin pathway. Dkks contain two characteristic cysteine-rich domains (CRD-1 and CRD-2) separated by a linker region of variable length. Two possible mechanisms by which Dkk1 inhibits the β-catenin pathway have been proposed. One is that Dkk1 prevents the formation of a ternary complex of Wnt, Fz and LRP6 on the cell surface by binding to LRP6 . In this mechanism, Dkk1 does not induce the internalization of LRP6 . Another possibility is that Dkk1 induces the internalization of LRP6 through a clathrin- mediated route, thus removing LRP6 from the plasma membrane

Related Publications:
the fanconi anemia pathway has a dual function in Dickkopf-1 transcriptional repression
PNAS, Feb 2014; 111: 2152 - 2157.
Dickkopf-1, the wnt-antagonist, is induced by acidic ph and mediates epithelial cellular senescence in human reflux esophagitis
Am J Physiol Gastrointest Liver Physiol, Jan 2014; 10.1152/ajpgi.00153.2013.
the relationship between inhibitors of the wnt signalling pathway (sclerostin and Dickkopf-1) and carotid intima-media thickness in postmenopausal women with type 2 diabetes mellitus
Diabetes and Vascular Disease Research, Jan 2014; 11: 48 - 52.
the association between serum Dickkopf-1 levels and esophageal squamous cell carcinoma
Human and Experimental Toxicology, Nov 2013; 10.1177/0960327113510537.
nitric oxide production upregulates wnt/β-catenin signaling by inhibiting Dickkopf-1
Cancer Res., Nov 2013; 73: 6526 - 6537.

by class:
Growth factors
CD markers
Viral Antigens
by species:
Purified Polyclonal
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product_icon_8.jpg Human Dickkopf-1 (DKK1) Recombinant
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