Cart contains    items
SEARCH

  |
  |  
  |  
  |  
  |  
  |  
 
Browse by molecule: [A]  [B]  [C]  [D]  [E]  [F]  [G]  [H]  [I]  [J]  [K]  [L]  [M]  [N]  [O]  [P]  [Q]  [R]  [S]  [T]  [U]  [V]  [W]  [X]  [Y]  [Z]  

Human Dickkopf-1 (DKK1) Recombinant

DKK1

accession:  O94907
   Size A     10ug   $ 160
   Size B      50ug  $480
   Size C      500ug  $ 3500
Domain  : 
Gene  :  DKK1
Catalog no. :  RKO94907

 

Source:

Optimized DNA sequence  encoding  extracellular domain of Human Dickkopf-1 (DKK-1)(THR32 - HIS266) including a C-terminal His tag was expressed in HEK293 cells.


 
Molecular weight:

Recombinant human DKK1 is a protein consisting of 240 amino acid residue subunits,due to glycosylation migrates as an approximately 40kDa protein on reduced SDS-PAGE.


 
Purity:
>95%, as determined by SDS-PAGE and HPLC
 
Biological Activity:
The activity was tested by the ability to inhibit Wnt3-ainduced alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells.
 
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant Human Dickkopf-1 is lyophilized  from 0.2 μm filtered PBS solution,  pH7.2 ,  5% Trehalose.

              
Storage:
Recombinant  DKK1 can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.

Avoid repeated freeze/thaw cycles.

 
Usage:
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

Dickkopf-1

The Dickkopf (Dkk) protein family, which comprises four members (Dkk1 to Dkk4), binds to LRP6 and antagonizes the β-catenin pathway. Dkks contain two characteristic cysteine-rich domains (CRD-1 and CRD-2) separated by a linker region of variable length. Two possible mechanisms by which Dkk1 inhibits the β-catenin pathway have been proposed. One is that Dkk1 prevents the formation of a ternary complex of Wnt, Fz and LRP6 on the cell surface by binding to LRP6 . In this mechanism, Dkk1 does not induce the internalization of LRP6 . Another possibility is that Dkk1 induces the internalization of LRP6 through a clathrin- mediated route, thus removing LRP6 from the plasma membrane

Related Publications:
the fanconi anemia pathway has a dual function in Dickkopf-1 transcriptional repression
PNAS, Feb 2014; 111: 2152 - 2157.
Dickkopf-1, the wnt-antagonist, is induced by acidic ph and mediates epithelial cellular senescence in human reflux esophagitis
Am J Physiol Gastrointest Liver Physiol, Jan 2014; 10.1152/ajpgi.00153.2013.
the relationship between inhibitors of the wnt signalling pathway (sclerostin and Dickkopf-1) and carotid intima-media thickness in postmenopausal women with type 2 diabetes mellitus
Diabetes and Vascular Disease Research, Jan 2014; 11: 48 - 52.
the association between serum Dickkopf-1 levels and esophageal squamous cell carcinoma
Human and Experimental Toxicology, Nov 2013; 10.1177/0960327113510537.
nitric oxide production upregulates wnt/β-catenin signaling by inhibiting Dickkopf-1
Cancer Res., Nov 2013; 73: 6526 - 6537.




by class:
Cytokines
Chemokines
Growth factors
CD markers
Viral Antigens
Other
by species:
Human
mouse
Rat
Antibodies:
Purified Polyclonal
Related Products:
product_icon_8.jpg Human Dickkopf-1 (DKK1) Recombinant, Biotinylated
product_icon_8.jpg Human Dickkopf-1 (DKK1) Recombinant
 
 
 
Item name Size Quantity Price(USD) Remove
checkout
Cart Total $