Optimized DNA sequence encoding extracellular domain of Human Dickkopf-1 (DKK-1)(THR32 - HIS266) including a C-terminal His tag was expressed in HEK293 cells.
Recombinant human DKK1 is a protein consisting of 240 amino acid residue subunits,due to glycosylation migrates as an approximately 40kDa protein on reduced SDS-PAGE.
>95%, as determined by SDS-PAGE and HPLC
The activity was tested by the ability to inhibit Wnt3-ainduced alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells.
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant Human Dickkopf-1 is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
Recombinant DKK1 can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.
Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
The Dickkopf (Dkk) protein
family, which comprises four
members (Dkk1 to Dkk4),
binds to LRP6 and
antagonizes the β-catenin
pathway. Dkks contain two
domains (CRD-1 and CRD-2)
separated by a linker region
of variable length. Two
possible mechanisms by which
Dkk1 inhibits the β-catenin
pathway have been proposed.
One is that Dkk1 prevents
the formation of a ternary
complex of Wnt, Fz and LRP6
on the cell surface by
binding to LRP6 . In this
mechanism, Dkk1 does not
induce the internalization
of LRP6 . Another
possibility is that Dkk1
induces the internalization
of LRP6 through a clathrin-
mediated route, thus
removing LRP6 from the
the fanconi anemia pathway has a dual function in Dickkopf-1 transcriptional repression
2152 - 2157.
Dickkopf-1, the wnt-antagonist, is induced by acidic ph and mediates epithelial cellular senescence in human reflux esophagitis
Am J Physiol Gastrointest Liver Physiol,
the relationship between inhibitors of the wnt signalling pathway (sclerostin and Dickkopf-1) and carotid intima-media thickness in postmenopausal women with type 2 diabetes mellitus
Diabetes and Vascular Disease Research,
48 - 52.
the association between serum Dickkopf-1 levels and esophageal squamous cell carcinoma
Human and Experimental Toxicology,
nitric oxide production upregulates wnt/β-catenin signaling by inhibiting Dickkopf-1 Cancer Res.,
6526 - 6537.