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Human EpCAM Recombinant, Biotinylated

EpCAM

accession:  P16422
   Size A     10ug   $ 340
   Size B      25ug  $590
   Size C      100ug  $ 1950
Domain  : 
Gene  :  EPCAM
Catalog no. :  RBP16422

 

Source:

Optimized DNA sequence  encoding  extracellular domain of Human Epithelial cell adhesion molecule (EpCAM)(GLN24 - LYS265)including a C-terminal His tag was expressed in HEK293 cells.

Recombinant Human EpCAM was further labeled by Biotin for detection by Avidin conjugated secondary probes.

 
Molecular weight:

Recombinant human EpCAM is a protein consisting of 249 amino acid residue subunits,due to glycosylation migrates as an approximately 35kDa protein on reduced SDS-PAGE.


 
Purity:
>95%, as determined by SDS-PAGE and HPLC
 
Applications:

ELISA,WB

Biotinylated recombinant EpCAM was designed to be used in single step detection in ELISA ,WB.

 
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant Human EpCAM is lyophilized  from 0.2 μm filtered PBS solution,  pH7.2 ,  5% Trehalose.

              
Storage:
Recombinant  EpCAM can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.

Avoid repeated freeze/thaw cycles.

 
Usage:
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

EpCAM

Epithelial cell adhesion molecule (EpCAM), also known as CD326, TACSTD1, and TROP- 1, is a type I transmembrane glycoprotein that was first identified as a tumor- associated antigen (1). EpCAM is highly expressed by many carcinomas, and its possible involvement in tumor development, progression, and metastasis has been extensively studied. EpCAM is also widely expressed by developing epithelia in normal individuals, and high levels persist in some epithelia in adults . Although EpCAM has been less extensively studied in normal tissues and nontransformed cells than in cancer, there are indications that EpCAM influences epithelial homeostasis. Expression of EpCAM promotes aggregation of fibroblasts in suspension, suggesting that EpCAM can function as an intercellular adhesion molecule

Related Publications:
internalized PCSK9 dissociates from recycling ldl receptors in PCSK9-resistant sv-589 fibroblasts
J. Lipid Res., Feb 2014; 55: 266 - 275.
identification of a small peptide that inhibits PCSK9 protein binding to the low density lipoprotein receptor
J. Biol. Chem., Jan 2014; 289: 942 - 955.
loss of plasma proprotein convertase subtilisin/kexin 9 (PCSK9) after lipoprotein apheresis
Circ. Res., Dec 2013; 113: 1290 - 1295.
characterization of the role of egf-a of low density lipoprotein receptor in PCSK9 binding
J. Lipid Res., Dec 2013; 54: 3345 - 3357.
role of PCSK9 and idol in the pathogenesis of acquired ldl receptor deficiency and hypercholesterolemia in nephrotic syndrome
Nephrol. Dial. Transplant., Oct 2013; 10.1093/ndt/gft439.




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