Human PD-L1 (B7-H1) Recombinant
Categories: CytokinesRecombinant Human Cytokines$160.00 – $1,950.00
Description
Accession
Q9EP73
Source
Optimized DNA sequence encoding extracellular domain of Human Programmed Death Ligand 1 ,PD-L1, (PHE19 - ARG238) including a C-terminal His tag was expressed in HEK293 cells.
Molecular weight
Recombinant human PD-L1 (B7-H1) is a protein consisting of 225 amino acid residue subunits,due to glycosylation migrates as an approximately 40kDa protein on reduced SDS-PAGE.
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
The activity was tested by the ability of immobilized recombinant Human PD-L1 to bind recombinant human PD1 within a linear range of 1-10ug/ml in ELISA.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human PD-L1 is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
Storage
Recombinant B7-H1 can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to-70°Cfor twelve months. Avoid repeated freeze/thaw cycles.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Molecular function
Methods
MPLA-tDCs exhibit a semi-mature surface phenotype and differentially express high levels of TLR-2.
- TLR-2, PD-L1, GILZ and ILT3 expression on each DC condition was analyzed by flow cytometry to seek for specific tolerogenic DC markers.
Expression of programmed death-1 (PD-1) in macrophages/microglia after spinal cord injury (SCI) in mice.
- Immunohistochemistry in macrophages/microglia 14 days postinjury (dpi) in wild-type mice for (c) PD-1, (d) it’s ligand, PD-L1, and (e) PD-1 in M1 cells [inducible nitric oxide synthase (iNOS)+]; (f) PD-1 in M2 cells [arginase 1 (Arg1+)]; (g) PD-L1 in M1 cells (iNOS+); and (h) PD-L1 in M2 cells (Arg1+).
Expression of programmed death-1 (PD-1) in macrophages/microglia after spinal cord injury (SCI) in mice.
- Immunohistochemistry in macrophages/microglia 14 days postinjury (dpi) in wild-type mice for (c) PD-1, (d) it’s ligand, PD-L1, and (e) PD-1 in M1 cells [inducible nitric oxide synthase (iNOS)+]; (f) PD-1 in M2 cells [arginase 1 (Arg1+)]; (g) PD-L1 in M1 cells (iNOS+); and (h) PD-L1 in M2 cells (Arg1+).
