//Biotinylated Human PD-1/PDCD1 Protein Recombinant

Biotinylated Human PD-1/PDCD1 Protein Recombinant

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$542.00$1,805.00

SKU: KTPV3P5HB

Description

Accession
Q15116
Source
DNA sequence encoding Human PD-1/PDCD1 including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight
Recombinant Human PD-1/PDCD1 is a protein consisting of amino acid residues [Leu25-Gln167] ,The protein has a predicted MW of 18.9 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human PD-1/PDCD1 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Biological Process

Methods

Biotinylated Human PD-1/PDCD1 Protein Tris-Bis PAGE

  • Biotinylated Human PD-1 on Tris-Bis PAGE under reduced. The purity is greater than 95%.

Biotinylated Human PD-1/PDCD1 Protein SEC-HPLC

  • The purity of Biotinylated Human PD-1 is greater than 95% as determined by SEC-HPLC.

Biotinylated Human PD-1/PDCD1 Protein ELISA Data

  • Immobilized Biotinylated Human PD-1, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-PD-1 Antibody, hFc Tag with the EC50 of 14.0ng/ml determined by ELISA.

Biotinylated Human PD-1/PDCD1 Protein SPR Data

  • Human PD-L1, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human PD-1, His-Avi Tag with an affinity constant of 2.356 μM as determined in SPR assay (Biacore T200).
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