Optimized DNA sequence Â encoding Â extracellular domain of Human CD3Î´ including a C-terminal His tag, were expressed in HEK293 cells.
Recombinant CD3Î´ Â is a Â protein consisting ofÂ Phe22-Ala125 amino acid residue subunits, Â due to glycosylation migrates as an approximately 18-25kDa protein on SDS-PAGE.
>95%, as determined by SDS-PAGE and HPLC
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/Âµg(1EU/Âµg).
Recombinant Human CD3Î´ Â is lyophilized from 0.2 Î¼m filtered PBS solution, Â pH7.2 , Â 5% Trehalose.
Â Â Â Â Â Â Â Â
Recombinant CD3 heterodimer can be stored in working aliquots at 2Â° - 8Â° C for one month, or at -20Â°C toÂ -70Â°CÂ for twelve months.
Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
he pre–T-cell receptor (TCR)
and the αβTCR are multimeric
protein complexes expressed
on the surface of developing
T cells at different stages
of their ontogeny in the
thymus. The pre-TCR complex
consists of a disulfide-
linked heterodimer of an
invariant pre-Tα (pTα) and a
variable TCRβ chain
with the CD3γ, δ, ϵ, and ζ
The overall structure and
functioning of the pre-TCR
and TCR complexes in mice
and humans are quite
similar. Despite this
similarity, ablation of the
highly related CD3δ and CD3γ
subunits has markedly
different effects in mice
the feature of cd3, cd3 and cd3 gene expression in benzene-exposed and benzene- poisoned workers
Blood (ASH Annual Meeting Abstracts),
different composition of the human and the mouse t cell receptor explains different phenotypes of cd3 and cd3 immunodeficiencies
J. Exp. Med.,
2537 - 2544.
a membrane-proximal tetracysteine motif contributes to assembly of cd3 and cd3 dimers with the t cell receptor
J. Biol. Chem.,
36977 - 36984.
overlapping functions of human cd3 and mouse cd3 in t-cell development revealed in a humanized cd3-deficient mouse
3420 - 3427.
biochemical evidence for the presence of a single cd3 and cd3 chain in the surface t cell receptor/cd3 complex
J. Biol. Chem.,
51068 - 51074.