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Human CD3εδ heterodimer Recombinant

CD3

accession:  P07766
   Size A     10ug   $ 160
   Size B      50ug  $480
   Size C      500ug  $ 3500
Domain  : 
Gene  :  CD3ed
Catalog no. :  RKK07766

 

Source:
Optimized DNA sequence  encoding  extracellular domain of Human CD3ε including a flag tag was co-expressed  with a DNA sequence encoding the extracellular domain of CD3δ including a C-terminal His tag, were expressed in HEK293 cells.

 
Molecular weight:

Recombinant CD3 ε- CD3δ  is a  heterodimer protein consisting of 270 amino acid residue subunits,  due to glycosylation migrates as an approximately 20kDa and as  26kDa bands on reduced SDS-PAGE.


 
Purity:
>97%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
The activity was tested by the ability of immobilized recombinant human CD3 epsilon-CD3 delta heterodimer to bind Human OKT3  with a linear range of 5-20 ng/ml

 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant Human CD3ε-CD3δ  is lyophilized from 0.2 μm filtered PBS solution,  pH7.2 ,  5% Trehalose.

              
Storage:
Recombinant CD3 heterodimer can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.

Avoid repeated freeze/thaw cycles.

 
Usage:
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

CD3 epsilon delta

nvariant CD3 subunit dimers (CD3εγ, CD3εδ, and CD3ζζ) are the signaling components of the αβ T cell receptor (TCR). Although CD3γ and CD3δ each dimerize with CD3ε, there are differential CD3 subunit requirements for receptor assembly and signaling among T lineage subpopulations, presumably mandated by structural differences. Although the CD3γ and the CD3δ subunits each pair with CD3ε, there are differences in CD3 subunit requirements for various T lineage populations and their developmental stages.

Related Publications:
structure of the chicken cd3/ heterodimer and its assembly with the αβt cell receptor
J. Biol. Chem., Jan 2014; 10.1074/jbc.M113.544965.
solution structure of the cd3 ectodomain and comparison with cd3 as a basis for modeling t cell receptor topology and signaling
PNAS, Nov 2004; 101: 16867 - 16872.
crystal structure of a human cd3-/ dimer in complex with a ucht1 single-chain antibody fragment
PNAS, Nov 2004; 101: 16268 - 16273.
normal development and function of natural killer cells in CD3 epsilon delta 5/delta 5 mutant mice.
PNAS, Aug 1995; 92: 7545 - 7549.




by class:
Cytokines
Chemokines
Growth factors
CD markers
Viral Antigens
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by species:
Human
mouse
Rat
Antibodies:
Purified Polyclonal
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