Optimized DNA sequence encoding extracellular domain of Human CD3ε including a flag tag was co-expressed with a DNA sequence encoding the extracellular domain of CD3δ including a C-terminal His tag, were expressed in HEK293 cells.
Recombinant CD3 ε- CD3δ is a heterodimer protein consisting of 270 amino acid residue subunits, due to glycosylation migrates as an approximately 20kDa and as 26kDa bands on reduced SDS-PAGE.
>97%, as determined by SDS-PAGE and HPLC
The activity was tested by the ability of immobilized recombinant human CD3 epsilon-CD3 delta heterodimer to bind Human OKT3 with a linear range of 5-20 ng/ml
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant Human CD3ε-CD3δ is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
Recombinant CD3 heterodimer can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.
Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
CD3 epsilon delta
nvariant CD3 subunit dimers
(CD3εγ, CD3εδ, and CD3ζζ)
are the signaling components
of the αβ T cell receptor
Although CD3γ and CD3δ each
dimerize with CD3ε, there
are differential CD3 subunit
requirements for receptor
assembly and signaling among
T lineage subpopulations,
presumably mandated by
Although the CD3γ and the
CD3δ subunits each pair with
CD3ε, there are differences
in CD3 subunit requirements
for various T lineage
populations and their
structure of the chicken cd3/ heterodimer and its assembly with the αβt cell receptor
J. Biol. Chem.,
solution structure of the cd3 ectodomain and comparison with cd3 as a basis for modeling t cell receptor topology and signaling
16867 - 16872.
crystal structure of a human cd3-/ dimer in complex with a ucht1 single-chain antibody fragment
16268 - 16273.
normal development and function of natural killer cells in CD3 epsilon delta 5/delta 5 mutant mice.
7545 - 7549.