Optimized DNA sequence encoding extracellular domain of Human CD3ε including a C-terminal His tag was expressed in HEK293 cells.
Recombinant CD3ε is a protein consisting of 116 amino acid residue subunits, due to glycosylation migrates as an approximately 20kDa protein on SDS-PAGE.
>97%, as determined by SDS-PAGE and HPLC
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant Human CD3ε is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
Recombinant CD3e can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.
Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Central to the function of
the immune system is the
ability of the TCR:CD3
complex to recognize and
bind to foreign peptides
presented by MHC molecules
and initiate an immune
response. The TCR is a
composed of at least eight
heterodimers of TCRαβ,
CD3εδ, and CD3εγ, and a
CD3ζζ homodimer, all of
which are necessary for
efficient TCR:CD3 surface