//Biotinylated Human Her2/ErbB2 Protein Recombinant

Biotinylated Human Her2/ErbB2 Protein Recombinant

Category:

$542.00$1,805.00

SKU: KTZHZH5HB

Description

Accession
P04626
Source
DNA sequence encoding Human Her2/ErbB2 including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight
Recombinant Human Her2/ErbB2 is a protein consisting of amino acid residues [Thr23-Thr652] ,The protein has a predicted MW of 72.3 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human Her2/ErbB2 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Interactor
Interactor
Interactor
Q13882
Interactor
Interactor
P62988
Interactor
Q93034
Biological Process
Molecular function
Molecular function
Molecular function

Methods

Biotinylated Human Her2/ErbB2 Protein Tris-Bis PAGE

  • Biotinylated Human Her2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Biotinylated Human Her2/ErbB2 Protein SEC-HPLC

  • The purity of Biotinylated Human Her2 is greater than 95% as determined by SEC-HPLC.

Biotinylated Human Her2/ErbB2 Protein ELISA Data

  • Immobilized Anti-Her2 Antibody, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human Her2, His Tag with the EC50 of 9.4ng/ml determined by ELISA.
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