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Rat Interleukin-20 Recombinant

IL-20

accession:  B8K1Y1
   Size A     10ug   $ 160
   Size B      50ug  $480
   Size C      1mg  $ 4300
Domain  :  four helix cytokine core
Gene  :  IL20
Catalog no. :  RKB8K1Y1
Source:
Optimized DNA sequence encoding Human Interleukin-20 mature chain was expressed in Escherichia Coli.

 
Molecular weight:

Native human Interleukin-20 generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 16kDa.

Recombinant IL-20 is a disulfide-linked monomeric protein consisting of  135 amino acid residue subunits, and migrates as an approximately 18kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.


 
Purity:
>95%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
The ED(50) was determined by by a cell proliferation assay using human IL-20Rα and human IL-20Rβ co-transfected murine BaF3 pro-B cells , and was found to be less than 0.6 ng/ml, corresponding to a specific activity of 1.7 × 10 6 IU/mg.  

 
Protein Sequence:
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant Human Interleukin-17 was lyophilized from a 0.2 μm filtered PBS solution pH 7.5.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

 

IL-20

L-19 and IL-20 are mainly produced by activated monocytes , IL-24 is produced by Th2 cells, monocytes, and melanocytic cells , and IL-22 and IL-26 are primarily produced by activated T cells . Like IL- 10, these class II cytokines signal through heterodimeric receptors. Overexpression of IL-20 in transgenic mice causes neonatal death as well as skin abnormalities, including aberrant epidermal differentiation. IL-20 selectively enhances multipotential hematopoietic progenitors in vitro and in vivo. IL-20 is preferentially expressed in monocytes18 and induces STAT 3 activation through binding to 2 types of IL-20 receptor (R) complexes, either IL- 20R1/IL-20R2 or IL-22R1/IL- 20R2

Related Publications:
anti–IL-20 monoclonal antibody alleviates inflammation in oral cancer and suppresses tumor growth
Mol. Cancer Res., Nov 2012; 10: 1430 - 1439.
anti–IL-20 monoclonal antibody suppresses breast cancer progression and bone osteolysis in murine models
J. Immunol., Feb 2012; 188: 1981 - 1991.
expression of IL-20 in lesional skin and synovium of patients with psoriatic arthritis compared to rheumatoid arthritis and its response to alefacept treatment
Ann Rheum Dis, Feb 2012; 71: A15.
anti–IL-20 monoclonal antibody inhibits the differentiation of osteoclasts and protects against osteoporotic bone loss
J. Exp. Med., Aug 2011; 208: 1849 - 1861.
IL-20 blockade prevents the upregulation of ccl2, ccl4, ccl5 and ccr7 by ra synovial biopsies
Ann Rheum Dis, Mar 2011; 70: A76 - A77.




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