Optimized DNA sequence encoding Human 4-1BB (CD137) fused to a C terminal IgG1 Fc tag was expressed in CHO Cells.
Recombinant Human 4-1BB (CD137) is a homodimer protein consisting of two 410 amino acid residue subunits, due to glycosylation migrates as an approximately 65kDa protein on SDS-PAGE under reducing conditions.
>97%, as determined by SDS-PAGE and HPLC
The activity was tested by the ability of immobilized recombinant mouse 4-1BBL to bind human 4-1BB with a linear range of 10-500 ng/ml
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant mouse CD137 is lyophilized from 0.2 μm filtered PBS solution, pH7.2 .
Recombinant Mouse 4-1BB can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.
Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
4-1BB (CD137) is a member of
the TNF receptor superfamily
expressed on activated CD4
and CD8 T cells as well as
on activated NK cells. The
binding of 4-1BB to its TNF
family ligand, 4-1BB ligand
(4-1BBL) delivers a
costimulatory signal to
resting T cells leading to
high level IL-2 production
independently of CD28
pilot study of redirected autologous t cells engineered to contain anti-cd19 attached to tcr and 4-1BB signaling domains in patients with chemotherapy-resistant or -refractory cd19+ leukemia and lymphoma.
ASCO Meeting Abstracts,
gitr-dependent regulation of 4-1BB expression: implications for t cell memory and anti–4-1BB–induced pathology
4627 - 4639.
leukocyte-specific protein 1 links tnf receptor-associated factor 1 to survival signaling downstream of 4-1BB in t cells
J. Leukoc. Biol.,
713 - 721.
systemic 4-1BB activation induces a novel t cell phenotype driven by high expression of eomesodermin
J. Exp. Med.,
743 - 755.
a converse 4-1BB and cd40 ligand expression pattern delineates activated regulatory t cells (treg) and conventional t cells enabling direct isolation of alloantigen-reactive natural foxp3+ treg
5985 - 5994.