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Human 4-1BB Ligand (TNFSF9) Recombinant

4-1BBL

accession:  P41273
   Size A     5ug   $ 70
   Size B      20ug  $160
   Size C      1mg  $ 2700
Domain  : 
Gene  :  TNFSF9
Catalog no. :  RKP41273
Source:
Optimized DNA sequence  encoding  Human 4-1BB Ligand (4-1BBL)  was expressed in E.COli.

 
Molecular weight:

Recombinant 4-1BBL  is a  protein consisting of 184 amino acid residue subunits,  due to glycosylation migrates as an approximately 20kDa protein on SDS-PAGE under reducing conditions.


 
Purity:
>97%, as determined by SDS-PAGE and HPLC

 
Sequence:

 

        10         20         30         40         50         60 
MEYASDASLD PEAPWPPAPR ARACRVLPWA LVAGLLLLLL LAAACAVFLA CPWAVSGARA

        70         80         90        100        110        120 
SPGSAASPRL REGPELSPDD PAGLLDLRQG MFAQLVAQNV LLIDGPLSWY SDPGLAGVSL 

       130        140        150        160        170        180 
TGGLSYKEDT KELVVAKAGV YYVFFQLELR RVVAGEGSGS VSLALHLQPL RSAAGAAALA 

       190        200        210        220        230        240 
LTVDLPPASS EARNSAFGFQ GRLLHLSAGQ RLGVHLHTEA RARHAWQLTQ GATVLGLFRV 

       250 
TPEIPAGLPS PRSE 

 


 
Biological Activity:
The activity was tested by the ability of recombinant human 4-1BBL to stimulate production of IL-8 in human PBMC  with a specific activity of  1x105 IU/mg

 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant Human 4-1BB Ligand  is lyophilized from 0.2 μm filtered PBS solution,  pH7.2 .

              
Storage:
Recombinant 4-1BBL can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.

Avoid repeated freeze/thaw cycles.

 
Usage:
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

4-1BBL

4-1BB (CD137) is a member of the TNF receptor superfamily expressed on activated CD4 and CD8 T cells as well as on activated NK cells. The binding of 4-1BB to its TNF family ligand, 4-1BB ligand (4-1BBL) delivers a costimulatory signal to resting T cells leading to high level IL-2 production independently of CD28 signaling .

Related Publications:
4-1BBL reverse signaling enhances proliferation of human multiple myeloma cell line u266
Blood (ASH Annual Meeting Abstracts), Nov 2011; 118: 5074.
the combination of 4-1BBL and cd40l strongly enhances the capacity of dendritic cells to stimulate hiv-specific t cell responses
J. Leukoc. Biol., Jun 2011; 89: 989 - 999.
4-1BBL-expressing aapcs attenuate il-15-induced nk cell expansion and cytokine production in vitro but induce nk cell-mediated gvhd in vivo
Blood (ASH Annual Meeting Abstracts), Nov 2010; 116: 2536.
abstract 2416: sa-4-1BBL costimulatory ligand as an immune modulator and effective vehicle to deliver antigens into dendritic cells for the generation of robust therapeutic antitumor immune responses
Cancer Res., Apr 2010; 70: 2416.
abstract 5595: antitumor immune responses to nitroreductase/cb1954 and 4-1BBL gene therapy
Cancer Res., Apr 2010; 70: 5595.




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Antibodies:
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Related Products:
product_icon_8.jpg Human 4-1BB Ligand (TNFSF9) Recombinant
product_icon_8.jpg Mouse 4-1BB Ligand (TNFSF9) Recombinant
 
 
 
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