Optimized DNA sequence encoding Human CD30 Ligand including an N-terminal IgG1 Fc tag was expressed in HEK293 cells.
Recombinant CD30 Ligand (CD30L) is a homodimer protein consisting of two 430 amino acid residue subunits, due to glycosylation migrates as an approximately 65kDa protein on SDS-PAGE under reducing conditions.
>97%, as determined by SDS-PAGE and HPLC
The activity was tested by the ability of immobilized recombinant human CD30L to bind Human CD30 with a linear range of 300-2000 pg/ml
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant Human CD30L is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
Recombinant CD30 can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.
Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
CD30 ligand (CD153) is a 40-
kDa type II membrane-
belonging to the TNF
superfamily and is expressed
on activated CD4+ Th cells
in addition to macrophages,
dendritic cells, B cells,
and unique CD4+CD3−CD11c−
accessory cells. CD30, the
receptor for CD30L (and
belonging to the TNFR
superfamily) was reported to
be preferentially expressed
by activated or memory Th
cells but not by resting B
or T cells.
CD30 Ligand is a target for a novel biological therapy against colitis associated with th17 responses
7671 - 7680.
combinatorial effects of CD30 Ligand stimulation or inhibitor of apoptosis (iap) antagonist exposure with conventional chemotherapy on cd30 positive malignancies
Blood (ASH Annual Meeting Abstracts),
CD30 Ligand/cd30 plays a critical role in th17 differentiation in mice
2222 - 2230.
a novel role of cd30/CD30 Ligand signaling in the generation of long-lived memory cd8+ t cells
4627 - 4634.
ox40 ligand and CD30 Ligand are expressed on adult but not neonatal cd4+cd3 inducer cells: evidence that il-7 signals regulate CD30 Ligand but not ox40 ligand expression
6686 - 6691.