Optimized DNA sequence encoding extracellular domain of Mouse L-Selectin including a C-terminal IgG1 Fc tag was expressed in HEK293 cells.
Recombinant L-Selectin is a homodimer protein consisting of 540 amino acid residue subunits, due to glycosylation migrates as an approximately 100kDa protein on SDS-PAGE.
>97%, as determined by SDS-PAGE and HPLC
The activity was tested by the ability of immobilized recombinant Mouse L-Selectin to bind Human U937 lymphoma cells
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant Human L-Selectin (CD62L) is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
Recombinant L-Selectin can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.
Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
lymphocyte binding to high
endothelial venules of
peripheral lymph nodes and
leukocyte interactions with
vascular endothelial cells
during immune and
inflammatory responses. L-
selectin is constitutively
expressed by most
leukocytes, whereas the
other members of the
selectin family, P- and E-
selectin, are expressed by
endothelium. In addition to
interactions, L-selectin can
also mediate intercellular
adherence to cultured
endothelial cells supports
the continued rolling of
newly arriving neutrophils
tweak inhibits traf2-mediated CD40 signaling by destabilization of CD40 signaling complexes
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inhibition of cd8+ t cell–derived CD40 signals is necessary but not sufficient for foxp3+ induced regulatory t cell generation in vivo
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effects of CD40 ligation combined with chemotherapy drugs on human breast cancer cell lines
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