//Biotinylated Human CD40/TNFRSF5 Protein Recombinant

Biotinylated Human CD40/TNFRSF5 Protein Recombinant

Category:

$542.00$1,805.00

SKU: KT2ZZRYHB

Description

Accession
P25942
Source
DNA sequence encoding Human CD40/TNFRSF5 including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight
Recombinant Human CD40/TNFRSF5 is a protein consisting of amino acid residues [Glu21-Arg193] ,The protein has a predicted MW of 22.1 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human CD40/TNFRSF5 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
O08863
Interactor
B2RUR8
Interactor
P39429
Interactor
Q13114
Interactor
Q60803
Interactor
Interactor
Q12933
Biological Process
Molecular function

Methods

Biotinylated Human CD40/TNFRSF5 Protein Tris-Bis PAGE

  • Biotinylated Human CD40 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Biotinylated Human CD40/TNFRSF5 Protein SEC-HPLC

  • The purity of Biotinylated Human CD40 is greater than 95% as determined by SEC-HPLC.

Biotinylated Human CD40/TNFRSF5 Protein ELISA Data

  • Immobilized Biotinylated Human CD40, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human CD40L, hFc Tag with the EC50 of 33ng/ml determined by ELISA.

Biotinylated Human CD40/TNFRSF5 Protein ELISA Data

  • Immobilized Biotinylated Human CD40, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-CD40 Antibody, hFc Tag with the EC50 of 9.9ng/ml determined by ELISA.

Biotinylated Human CD40/TNFRSF5 Protein Blocking Data

  • Serial dilutions of Anti-CD40 Antibody were added into Biotinylated Human CD40, His Tag : Human CD40 Ligand (Trimer) , hFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 2.0ug/ml.
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