Optimized DNA sequence encoding extracellular domain of Human CD40 including a C-terminal His tag was expressed in HEK293 cells.
Recombinant CD40 is a protein consisting of 180 amino acid residue subunits, due to glycosylation migrates as an approximately 30kDa protein on SDS-PAGE.
>97%, as determined by SDS-PAGE and HPLC
The activity was tested by the ability of immobilized recombinant human CD40 to bind Human CD40 Ligand with a linear range of 10-500 ng/ml
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant Human CD40 is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Recombinant CD40 can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.
Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
The CD40 molecule is a 45-
50-kDa type I phosphorylated
glycoprotein that belongs to
the tumor necrosis factor
receptor superfamily and was
initially considered to be a
growth factor receptor on B
cells. The interaction of
CD40 with its natural
ligand, CD154, is a crucial
step in T cell-dependent B
cell activation and
The cellular events
triggered in CD40-positive
cells following CD40/CD154
interactions are not
restricted to B cells only
but can also be triggered in
dendritic cells, monocytes,
endothelial cells, and
fibroblasts. Thus, CD40
ligation mediates a broad
variety of immune and
inflammatory responses .
tweak inhibits traf2-mediated CD40 signaling by destabilization of CD40 signaling complexes
2308 - 2318.
a phase i study of an agonist CD40 monoclonal antibody (cp-870,893) in combination with gemcitabine in patients with advanced pancreatic ductal adenocarcinoma
Clin. Cancer Res.,
inhibition of cd8+ t cell–derived CD40 signals is necessary but not sufficient for foxp3+ induced regulatory t cell generation in vivo
1957 - 1964.
modulation of regulatory t cell function by monocyte-derived dendritic cells matured through electroporation with mrna encoding CD40 ligand, constitutively active tlr4, and cd70
1976 - 1983.
effects of CD40 ligation combined with chemotherapy drugs on human breast cancer cell lines
Journal of International Medical Research,