Biotinylated Human CD30/TNFRSF8 Protein Recombinant
Category: Biotinylated proteins$542.00 – $1,805.00
Description
Accession
P28908
Source
DNA sequence encoding Human CD30/TNFRSF8 including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight
Recombinant Human CD30/TNFRSF8 is a protein consisting of amino acid residues [Phe19-Lys379] ,The protein has a predicted MW of 41.3 kDa. Due to glycosylation, the protein migrates to 70-100 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human CD30/TNFRSF8 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Molecular function
Methods
Biotinylated Human CD30/TNFRSF8 Protein Tris-Bis PAGE
- Biotinylated CD30 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Biotinylated Human CD30/TNFRSF8 Protein SEC-HPLC
- The purity of Biotinylated CD30 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human CD30/TNFRSF8 Protein ELISA Data
- Immobilized Anti-CD30 Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human CD30, His Tag with the EC50 of 49ng/ml determined by ELISA.
Biotinylated Human CD30/TNFRSF8 Protein
- Immobilized Human CD30 Ligand, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human CD30, His Tag with the EC50 of 27.1ng/ml determined by ELISA.
