Optimized DNA sequence encoding extracellular domain of Mouse IL-18 receptor including an C terminal IgG1 Fc tag was expressed in HEK293 cells.
Recombinant mouse IL-18 receptor alpha is a homodimer protein consisting of two 555 amino acid residue subunits, due to glycosylation migrates as an approximately 95kD protein under reducing SDS-PAGE conditions.
>98%, as determined by SDS-PAGE and HPLC
Measured by its ability to bind immobilized recombinant human IL-18 at a concentration range of 1-32ng/ml in a functional ELISA assay.
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant Mouse Interleukin-18 receptor alpha is supplied as lyophilized 0.2 μm filtered PBS solution, pH7.2 .
Recombinant IL-18 receptor alpha, as supplied, can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.
Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
The IL-18R complex is
composed of two known
chains, IL-18 receptor α
chain (IL-18Rα)and IL-18Rβ.
IL-18Rα (IL-1R5) was
originally described as IL-1
(IL-1Rrp) because of its
homology with the IL-1/Toll
receptor family and is the
extracellular binding domain
of the IL-18R complex. IL-
18Rβ (also known as IL-
18RAcPL or IL-1R7) is the
signal transducing chain of
the IL-18R complex and is
also a member of the IL-1R
family. It has been widely
believed that the IL-18Rα is
not expressed on naive T
cells, but is induced during
differentiation of Th1, but
not Th2, cells.
the interaction between il-18 and IL-18 receptor limits the magnitude of protective immunity and enhances pathogenic responses following infection with intracellular bacteria
1333 - 1346.
the natural soluble form of IL-18 receptor β exacerbates collagen-induced arthritis via modulation of t-cell immune responses
Ann Rheum Dis,
276 - 283.
il-18 is induced and IL-18 receptor plays a critical role in the pathogenesis of cigarette smoke-induced pulmonary emphysema and inflammation
1948 - 1959.
responses of il-18- and IL-18 receptor-deficient pancreatic islets with convergence of positive and negative signals for the IL-18 receptor PNAS,
16852 - 16857.
blockade of IL-18 receptor signaling delays the onset of autoimmune disease in mrl-faslpr mice
5312 - 5318.