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Mouse CD34 Recombinant

CD34

accession:  Q64314
   Size A     10ug   $ 160
   Size B      50ug  $550
   Size C      500ug  $ 3500
Domain  : 
Gene  : 
Catalog no. :  RKQ64314
Source:
Optimized DNA sequence encoding extracellular domain of mouse CD34 including a C-terminal 6His tag was expressed in HEK293 cells.

 
Molecular weight:
Recombinant mouse CD34 is a monomer protein consisting of 270 amino acid residue subunits, due to glycosylation migrates as an approximately 50 kDa protein on SDS-PAGE.
 

 
Purity:
>95%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
Measured by the ability to support the adhesion of the HUVEC cell line. Coated plates with immobilized recombinant mouse CD34 ( 0.8 μg/mL) are able to adhere 40% of cells ( 4 x 104 cells/well) after one hour at 37 ℃
 

 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant mouse CD34 is supplied as a 0.2 μm filtered PBS solution, pH7.2.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:

This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

 

CD34

CD34 is a transmembrane glycoprotein expressed by human hematopoietic progenitor cells of lymphoid and myeloid lineage, and various types of colony-forming activity in human bone marrow (BM), cord blood (CB), and mobilized peripheral blood (PB) are contained in the CD34+ population. It has been suggested that CD34 also defines a normally occurring population of dendritic cells within the endoneurium of peripheral nerves that is distinct from fibroblasts and conventional Schwann cells

Related Publications:
loss of CD34 leads to exacerbated autoimmune arthritis through increased vascular permeability
J. Immunol., Feb 2010; 184: 1292 - 1299.
a gene expression signature of CD34+ cells to predict major cytogenetic response in chronic-phase chronic myeloid leukemia patients treated with imatinib
Blood, Jan 2010; 115: 315 - 325.
in vitro–differentiated t/natural killer–cell progenitors derived from human CD34+ cells mature in the thymus
Blood, Jan 2010; 115: 261 - 264.
human CD34+ cells engineered to express membrane-bound tumor necrosis factor-related apoptosis-inducing ligand (trail) target both tumor cells and tumor vasculature
Blood, Jan 2010; 10.1182/blood-2009-08-239632.
leukemia initiating cells from some acute myeloid leukemia patients with mutated nucleophosmin reside in the CD34- fraction
Blood, Jan 2010; 10.1182/blood-2009-02-206565.




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Antibodies:
Purified Polyclonal
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