Optimized DNA sequence encoding extracellular domain of human CD31 including a C-terminal 6His tag was expressed in HEK293 cells.
Recombinant CD31 is a homodimer protein consisting of 812 amino acid residue subunits, due to glycosylation migrates as an approximately 140 kDa protein on SDS-PAGE.
>95%, as determined by SDS-PAGE and HPLC
Recombinant Human CD31 activity was determined by its ability to support the adhesion of Jurkat human leukemic T cells. When cells are added to recombinant human CD31 coated plates ( 3.3 μg/mL , 100 μL/well ) in the presence of PMA , approximately 50% - 65% will adhere specifically after 30 minutes at 37℃
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant human CD31 is supplied as a 0.2 μm filtered PBS solution, pH7.2 .
Recombinant CD31, as supplied, can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.
Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
adhesion molecule 1, also
known as CD31, is a member
of the Ig gene superfamily
expressed at high density at
the lateral borders of
endothelial cells and at a
lower density on the surface
of hematopoietic cells
including T lymphocytes. By
endothelium- and leukocyte-
expressed CD31, this
molecule has been shown to
(TEM) in vitro and in vivo.
cd38/CD31, the ccl3 and ccl4 chemokines, and cd49d/vascular cell adhesion molecule-1 are interchained by sequential events sustaining chronic lymphocytic leukemia cell survival
4001 - 4009.
circulating monocytes expressing CD31: implications for acute and chronic angiogenesis
Am. J. Pathol.,
1972 - 1980.
il-7 sustains CD31 expression in human naive cd4+ t cells and preferentially expands the CD31+ subset in a pi3k-dependent manner
2999 - 3007.
transcriptional profiling of CD31+ cells isolated from murine embryonic stem cells
243 - 260.
life after the thymus: CD31+ and CD31– human naive cd4+ t-cell subsets
769 - 774.