Optimized DNA sequence encoding extracellular domain of mouse CD25 including a C-terminal polyHis tag was expressed in HEK293 cells.
Recombinant mouse CD25 is a monomer protein consisting of 223 amino acid residue subunits, due to glycosylation migrates as an approximately 50 kDa protein on SDS-PAGE.
>97%, as determined by SDS-PAGE and HPLC
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant CD25 is supplied as a 0.2 μm filtered PBS solution, pH7.2 .
Recombinant CD25, as supplied, can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.
Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
This cell surface antigen is
known also as TAC antigen .
It is expressed on T-cells
and B-cells following cell
activation by antigens. An
old designation for the
murine counterpart is Ly43 .
The rat antigen is OX39.
CD25 is identical with IL2RA
, which is the approved gene
symbol. It is called also T-
cell growth factor receptor
defects in il-2r signaling contribute to diminished maintenance of foxp3 expression in cd4+CD25+ regulatory t-cells of type 1 diabetic subjects
407 - 415.
il-2/anti-il-2 antibody complexes show strong biological activity by avoiding interaction with il-2 receptor subunit CD25 PNAS,
functional characterization of alloreactive t cells identifies CD25 and cd71 as optimal targets for a clinically applicable allodepletion strategy
396 - 407.
cd4+ t cell regulation of CD25 expression controls development of short-lived effector cd8+ t cells in primary and secondary responses
193 - 198.
cd4+CD25+ regulatory t cells resist a novel form of cd28- and fas-dependent p53-induced t cell apoptosis
94 - 104.