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Mouse Interleukin-21 Recombinant

IL-21

accession:  Q9ES17
   Size A     5ug   $ 160
   Size B      20ug  $480
   Size C      1mg  $ 4700
Domain  : 
Gene  :  IL21
Catalog no. :  RKQ9ES17
Source:
Optimized DNA sequence encoding Human Interleukin-21 mature chain was expressed in Escherichia Coli.

 
Molecular weight:
Mouse Interleukin-21 is generated by the proteolytic removal of the signal peptide
and propeptide, the molecule has a calculated molecular mass of approximately 15 kDa.

Recombinant Mouse IL-21 a disulfide-linked monomeric protein consisting of 123 amino acid residue subunits, and migrates as an approximately 15 kDa protein under non-reducing and reducing conditions in SDS-PAGE.


 
Purity:
>97%, as determined by SDS-PAGE and HPLC

 
 

 
Protein Sequence:
        10         20         30         40         50         60 
MERTLVCLVV IFLGTVAHKS SPQGPDRLLI RLRHLIDIVE QLKIYENDLD PELLSAPQDV 

        70         80         90        100        110        120 
KGHCEHAAFA CFQKAKLKPS NPGNNKTFII DLVAQLRRRL PARRGGKKQK HIAKCPSCDS 

       130        140 
YEKRTPKEFL ERLKWLLQKM IHQHLS 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant Interleukin-21 was lyophilized from a 0.2 μm filtered PBS solution pH 7.5.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

IL-21

IL21 stimulates proliferation of B-cell stimulated by crosslinking of the CD40 antigen. It inhibits proliferation stimulated by IL4 plus anti- IgM. IL21 augments stimulation of the proliferation of naive CD45RA(+) but not memory CD45R0(+) T-cells mediated by engagement of CD3. IL21 stimulates the proliferation of bone marrow progenitor cells and the expression of the NK-cell marker CD56 in the presence of IL15. The Interleukin-21 receptor is closely related to human IL2 beta receptor. The receptor contains a WSXWS motif in the extracellular region, typical of type 1 cytokine receptors. The receptor is expressed on NK-cells, T- cells, and B-cell line.The functional signaling complex activates Janus kinases JAK1, JAK3, and the STAT proteins STAT1, and STAT3.

Related Publications:
tlr3 ligand polyinosinic:polycytidylic acid induces il-17a and IL-21 synthesis in human th cells
J. Immunol., Oct 2009; 183: 4422 - 4431.
IL-21 and il-10 have redundant roles but differential capacities at different stages of plasma cell generation from human germinal center b cells
J. Leukoc. Biol., Sep 2009; 10.1189/jlb.0409268.
modulation of single-cell igg secretion frequency and rates in human memory b cells by cpg dna, cd40l, IL-21, and cell division
J. Immunol., Sep 2009; 183: 3177 - 3187.
induction of granulysin in cd8+ t cells by IL-21 and il-15 is suppressed by human immunodeficiency virus-1
J. Leukoc. Biol., Aug 2009; 10.1189/jlb.0409222.
IL-21 contributes to jak3/stat3 activation and promotes cell growth in alk-positive anaplastic large cell lymphoma
Am. J. Pathol., Aug 2009; 175: 825 - 834.




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