Human IL-7 receptor Recombinant (CD127)
Category: Surface & Soluble Receptors$160.00 – $1,950.00
Description
Accession
P16871
Source
Optimized DNA sequence encoding extracellular domain of Human IL-7 receptor alpha including a C-terminal His tag was expressed in HEK293 cells.
Molecular weight
Recombinant Human IL-7 receptor alpha (CD127) is a protein consisting of 226 amino acid residue subunits, due to glycosylation migrates as an approximately45 kDa protein on SDS-PAGE.
Purity
>97%, as determined by SDS-PAGE and HPLC
Biological Activity
Recombinant Human IL-7 receptor alpha (CD127) Activity was determined by its binding ability of biotinylated mouse TSLP in a functional ELISA.
Presentation
Recombinant Human IL-7R (CD127) is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
Storage
Recombinant Human IL-7 receptor can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to-70°Cfor twelve months. Avoid repeated freeze/thaw cycles.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Molecular function
Methods
Bioinformatic analysis for prediction of sCD127 glycation sites and experimental glycation of sCD127.
-
ecombinant human sC127 was experimentally glycated by incubation for 7 days in the dark at 37°C in the presence of 100 mmol/L
d -glucose (Sigma-Aldrich) or PBS as control. - sCD127 glycation was evaluated by nontryptophan fluorescence as previously reported (
