Optimized DNA sequence encoding Human extracellular domain of mouse IL-1 receptor type 1 including a C-terminal His tag was expressed in HEK293 cells.
Recombinant IL-R1 (CD121a) is a protein consisting of 330 amino acid residue subunits, due to glycosylation migrates as an approximately 55-60 kDa protein on SDS-PAGE.
>97%, as determined by SDS-PAGE and HPLC
Recombinant mouse IL1R1 activity was measured by its ability to bind mouse IL1B in a functional ELISA. Immobilized mouse IL1A at 10 μg/mL (100 μl/well) can bind mouse IL1R1, The EC50 of mouse IL1R1 is 0.13 μg/mL.
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant Mouse IL-1R (CD121a) is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
Recombinant mouse IL-1 receptor type 1 can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.
Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
pellino 2 is critical for toll-like receptor/interleukin-1 receptor (tlr/IL-1R)-mediated post-transcriptional control
J. Biol. Chem.,
25686 - 25695.
role of toll interleukin-1 receptor (IL-1R) 8, a negative regulator of IL-1R/toll-like receptor signaling, in resistance to acute pseudomonas aeruginosa lung infection
100 - 109.
IL-1R–associated kinase-1 mediates protein kinase c-induced il-1β production in monocytes
2632 - 2645.
effect of mitomycin c on IL-1R expression, il-1–related hepatocyte growth factor secretion and corneal epithelial cell migration
Invest. Ophthalmol. Vis. Sci.,
1389 - 1396.
mycobacterium bovis bacillus calmette-guérin vaccination mobilizes innate myeloid-derived suppressor cells restraining in vivo t cell priming via IL-1R–dependent nitric oxide production
2038 - 2047.