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Mouse TNFR1 Recombinant (CD120a)

TNFR1

accession:  P25118
   Size A       $
   Size B      50ug  $350
   Size C      500ug  $ 1950
Domain  :  Ig-like C2
Gene  :  TNFR2
Catalog no. :  RKP25118

Source:
Optimized DNA sequence  encoding  extracellular domain of Mouse TNF receptor type 1 (TNFR1)  including a C-terminal 6His tag was expressed in HEK293 cells.

 
Molecular weight:

Recombinant Mouse TNF receptor 1 (CD120a) is a protein consisting of 201 amino acid residue subunits, due to glycosylation migrates as an approximately 35kDa protein on SDS-PAGE.


 
Purity:
>95%, as determined by SDS-PAGE and HPLC
 

 
Biological Activity:

Recombinant Mouse TNF receptor 1 (CD120a) activity was determined by the ability to inhibit the TNFα mediated cytotoxicity in the L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D.

The ED50 for this effect is typically 0.5-1.5 μg/mL in the presence of 0.15 ng/mL recombinant mouse TNFα .

 

Endotoxin:

Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant Mouse TNFR1 (TNFRSF1A) is supplied as a 0.2 μm filtered PBS solution, pH7.2.

              
Storage:
Recombinant Mousen TNF receptor 1 (CD120a), as supplied, can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.

Avoid repeated freeze/thaw cycles.

 
Usage:
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

TNFR1

The effects of TNFα are mediated by two distinct cell surface receptors, p55 TNF-R1 and p75 TNF-R2, that are expressed on almost all nucleated cells. Both receptors lack recognizable enzymatic domains, and their ability to transduce signals is dependent on the interaction with proteins associating with their cytoplasmic tails. Like other members of the tumor necrosis factor (TNF) receptor family, p55 TNF receptor 1 (TNF-R1) lacks intrinsic signaling capacity and transduces signals by recruiting associating molecules. The TNF-R1 associated death domain protein interacts with the p55 TNF-R1 cytoplasmic domain and recruits the Fas- associated death domain protein.

Related Publications:
TNFR1-activated reactive oxidative species signals up-regulate osteogenic msx2 programs in aortic myofibroblasts
Endocrinology, Aug 2012; 153: 3897 - 3910.
TNFR1-dependent pulmonary apoptosis during ischemic acute kidney injury
Am J Physiol Lung Cell Mol Physiol, Jun 2012; 10.1152/ajplung.00301.2011.
apoptosis and expression of antiviral response genes during ocular hsv-1 infection in TNFR1 or tnfr2 knockout mice
Invest. Ophthalmol. Vis. Sci., Mar 2012; 53: 6158.
association of tnfα, TNFR1, and tnfr2 polymorphisms with sperm concentration and motility
J Androl, Jan 2012; 33: 74 - 80.
st6gal-i regulates macrophage apoptosis via α2-6 sialylation of the TNFR1 death receptor
J. Biol. Chem., Nov 2011; 286: 39654 - 39662.




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Antibodies:
Purified Polyclonal
Related Products:
product_icon_8.jpg Human TNFR1 Recombinant (CD120a)
product_icon_8.jpg Mouse TNFR1 Recombinant (CD120a)
 
 
 
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