Optimized DNA sequence encoding extracellular domain of mouse CSF-1 receptor (CSF-1R) including a C-terminal polyHis tag was expressed in HEK293 cells.
Recombinant mouse CSF-1 receptor is a monomer protein consisting of 504 amino acid residue subunits, due to glycosylation migrates as an approximately 90 kDa protein on SDS-PAGE.
>98%, as determined by SDS-PAGE and HPLC
Activity of recombinant mouse CSF-1 receptor was measured via its ability to bind biotinylated recombinant human M-SCF. The ED50 of biotinylated human M-CSF is 0.5 ug/mL
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant mouse CSF-1R is supplied as lyophilized 0.2 μm filtered PBS solution, pH7.2 .
Recombinant CSF-1R, as supplied, can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.
Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
The CSF-1R is a member of
the type III receptor
tyrosine kinase family that
includes other structurally
related kinases such as Kit
and platelet-derived growth
factor (PDGF)-R . CSF-1
binding to the CSF-1R
activation of key signaling
pathways including the PI-3
kinase/Akt and mitogen-
activated protein kinase
macrophage proliferation is regulated through CSF-1 receptor tyrosines 544, 559, and 807
J. Biol. Chem.,
13694 - 13704.
therapeutic applications of macrophage colony-stimulating factor-1 (csf-1) and antagonists of CSF-1 receptor (csf-1r) signaling
1810 - 1820.
autocrine csf-1 and CSF-1 receptor coexpression promotes renal cell carcinoma growth
187 - 200.
a CSF-1 receptor phosphotyrosine 559 signaling pathway regulates receptor ubiquitination and tyrosine phosphorylation
J. Biol. Chem.,
952 - 960.
functional overlap but differential expression of csf-1 and il-34 in their CSF-1 receptor-mediated regulation of myeloid cells
J. Leukoc. Biol.,
495 - 505.