Optimized DNA sequence encoding extracellular domain of human CD113 (nectin-3) including a C-terminal 6His tag was expressed in HEK293 cells.
Recombinant CD113 (nectin-3) is a monomer protein consisting of 354 amino acid residue subunits, due to glycosylation migrates as an approximately 70 kDa protein on SDS-PAGE.
>95%, as determined by SDS-PAGE and HPLC
The activity of Recombinant human CD113 was measured in a functional ELISA assay, immobilized recombinant human CD113 at 1μg/ml ( 100μl/well ) can bind biotinylated Nectin-1 with a linear range of 6 - 800 μg/ml
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant CD113 is supplied as a 0.2 μm filtered PBS solution, pH7.2.
Recombinant CD113, as supplied, can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.
Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Nectins are a small family
cell adhesion molecules
(CAMs) that control cell
adhesion, proliferation, and
is a 60 or 65 kDa type I TM
glycoprotein that is found
on a variety of cell types.
is known to bind the
virus, and herpes simplex
It does not bind poliovirus.
As a cell
adhesion molecule, Nectin2
will form cishomodimers
, followed by transdimers
inhibition of cell movement and proliferation by cellcell contact-induced interaction of necl-5 with Nectin-3 J. Cell Biol.,
165 - 173.
roles of cell-adhesion molecules nectin 1 and nectin 3 in ciliary body development
1525 - 1537.
Nectin-3 suppresses the invasive nature of human prostate cancer cells
AACR Meeting Abstracts,
nectin-like molecule-5/tage4 enhances cell migration in an integrin-dependent, Nectin-3-independent manner
J. Biol. Chem.,
18015 - 18025.
recruitment of Nectin-3 to cell-cell junctions through trans-heterophilic interaction with cd155, a vitronectin and poliovirus receptor that localizes to v3 integrin-containing membrane microdomains
J. Biol. Chem.,
31251 - 31260.