Optimized DNA sequence encoding extracellular domain of mouse CD118 (LIFR) including a C-terminal 6His tag was expressed in HEK293 cells.
Recombinant CD118 (LIF receptor) is a monomer protein consisting of 796 amino acid residue subunits, due to glycosylation migrates as an approximately 110 kDa protein on SDS-PAGE.
>90%, as determined by SDS-PAGE and HPLC
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant mouse CD118 (LIFR) is supplied as a 0.2 μm filtered PBS solution, pH7.2 .
Recombinant mouse CD118, as supplied, can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.
Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
LIF acts on its target cells
via the activation of the
LIF-receptor (LIFR) complex.
The LIFR complex requires
heterodimerisation of the
two low affinity components
LIFR and glycoprotein 130
(gp130), where combination
transforms a low-affinity
binding complex into a high-
affinity binding site.
Activation of the LIFR
complex can trigger several
signal transduction paIn
heterodimerization with the
LIFR, only the CBM of gp130
contacts LIF . The LIFR
contributes mainly with its
Ig-like domain to LIF
exercise rescued chronic kidney disease by attenuating cardiac hypertrophy through the cardiotrophin-1 LIFR/gp 130 jak/stat3 pathway
European Journal of Cardiovascular Prevention & Rehabilitation,
role of LIFR-gp130 activation in endogenous neuroprotection of retinal photoreceptors
Invest. Ophthalmol. Vis. Sci.,
dimerization of the cytokine receptors gp130 and LIFR analysed in single cells
J. Cell Sci.,
5129 - 5140.
the developmental expression of vasoactive intestinal peptide (vip) in cholinergic sympathetic neurons depends on cytokines signaling through LIFRß-containing receptors
1387 - 1396.
preimplantation embryology: expression of leukaemia inhibitory factor receptor subunits LIFRß and gp 130 in human oocytes and preimplantation embryos
Mol. Hum. Reprod.,
355 - 360.