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mouse Prolactin Recombinant

Prolactin

accession:  P06879
   Size A     2ug   $ 70
   Size B      10ug  $160
   Size C      1mg  $ 4700
Domain  :  four helix cytokine core
Gene  :  PRL
Catalog no. :  RKP068791
Source:
Optimized DNA sequence encoding Mouse Prolactin mature chain fused to a C terminal polyHis tag was expressed in Insect Cells.

 
Molecular weight:

Native mouse Prolactin is generated by the proteolytic removal of the signal peptide
and propeptide, the molecule has a calculated molecular mass of approximately 23 kDa.

Recombinant Prolcatin is a disulfide-linked monomeric protein consisting of 207 amino acid residue subunits, and migrates as an approximately 26 kDa protein under non-reducing conditions in SDS-PAGE.


 
Purity:
>95%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
The activity was determined by the dose-dependent  proliferation of Nb2 cells.

 
Protein Sequence:
        10         20         30         40         50         60 
MNSQGSAQKA GTLLLLLISN LLFCQNVQPL PICSAGDCQT SLRELFDRVV ILSHYIHTLY 

        70         80         90        100        110        120 
TDMFIEFDKQ YVQDREFMVK VINDCPTSSL ATPEDKEQAL KVPPEVLLNL ILSLVQSSSD 

       130        140        150        160        170        180 
PLFQLITGVG GIQEAPEYIL SRAKEIEEQN KQLLEGVEKI ISQAYPEAKG NGIYFVWSQL 

       190        200        210        220 
PSLQGVDEES KILSLRNTIR CLRRDSHKVD NFLKVLRCQI AHQNNC 
(*)Complete precursor sequence shown, expressed chain highlighted 
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant mouse Prolactin is presented as concentrated 1mg/ml, 0.2 μm filtered 20mM Tris solution,0.5M NaCl pH 7.4 containing 5% trehalose 10% glycerol.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration  of 0.1 mg/mL. 

 
Storage:
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

 

Prolactin

Prolactin is a neuroendocrine pituitary hormone of 23 kDa. It is produced in increasing amounts during pregnancy and during suckling and acts primarily on the mammary gland by initiating and maintaining lactation in the postpartal phase. rolactin has been shown also to have cytokine-like activities and to have important immunoregulatory activities. It contributes to the development of lymphoid tissues and the maintenance of physiological immune function and also modulates a variety of T- cell immune responses.In addition to triggering resting lymphocytes to cell division, the hormone can also control the magnitude of their response to polyclonal stimuli. Prolactin promotes the proliferation of Nb2 pre-T- cell lymphoma cells. In these cells prolactin induces the biphasic expression of a transcription factor, IRF1 and may be involved in Cell cycle activation and S phase progression.Prolactin has been reported to activate cellular proliferation in nonreproductive tissue, such as liver, spleen, and thymus. It induces significant proliferation in aortic smooth muscle cells and also enhances proliferation of these cells induced by PDGF.

Related Publications:
improving the pharmacokinetics/pharmacodynamics of Prolactin, growth hormone, and their antagonists by fusion to a synthetic albumin binding peptide
J. Endocrinol., Sep 2009; 10.1677/JOE-09-0211.
Prolactin regulation of testosterone secretion and testes growth in dls rams at the onset of seasonal testicular recrudescence
Reproduction, Sep 2009; 10.1530/REP-09-0180.
maternal Prolactin inhibition during lactation programs for metabolic syndrome in adult progeny
J. Physiol., Sep 2009; 10.1113/jphysiol.2009.176289.
the role of insulin-like growth factor 1 on the differentiation of Prolactin secreting cells in the mouse anterior pituitary
J. Endocrinol., Sep 2009; 10.1677/JOE-09-0232.
two-step stimulation of intestinal ca2+ absorption during lactation by long-term Prolactin exposure and suckling-induced Prolactin surge
Am J Physiol Endocrinol Metab, Sep 2009; 297: E609 - E619.




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