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Mouse Interleukin-18 Binding Protein (IL-18BP)Recombinant

IL-18

accession:  Q9Z0M9
   Size A     50ug   $ 160
   Size B      200ug  $480
   Size C      1mg  $ 1950
Domain  :  Ig Like C2
Gene  :  IL18
Catalog no. :  RKQ9Z0M9
Source:
Optimized DNA sequence encoding Mouse Interleukin-18 Binding Protein (IL-18BP) mature chain fused to an C-terminal polyHis tag was expressed in HEK cells.

 
Molecular weight:
Native Mouse IL-18BP, generated by the proteolytic removal of the signal peptide and propeptide,the molecule has a calculated molecular mass of approximately 19 kDa.

Recombinant Interleukin-18 Binding Protein is a disulfide-linked monomer protein consisting of 165 amino acid residue subunits fused to a  polyHis tag, migrates due to glycosylation as an approximately as a 35-45kDa protein under non-reducing and reducing conditions in SDS-PAGE.


 
Purity:
>95%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
The activity of recombinant Interleukin-18 Binding Protein was determined by the ability to bind IL-18 in functional ELISA.

 
Protein Sequence:
        10         20         30         40         50         60 
MTMRHCWTAG PSSWWVLLLY VHVILARATS APQTTATVLT GSSKDPCSSW SPAVPTKQYP 

        70         80         90        100        110        120 
ALDVIWPEKE VPLNGTLTLS CTACSRFPYF SILYWLGNGS FIEHLPGRLK EGHTSREHRN 

       130        140        150        160        170        180 
TSTWLHRALV LEELSPTLRS TNFSCLFVDP GQVAQYHIIL AQLWDGLKTA PSPSQETLSS 

       190 
HSPVSRSAGP GVA 
(*)Complete precursor sequence shown, expressed chain highlighted
 

 

Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant IL-18BP is supplied as an 0.2 μm filtered PBS solution.

 
Reconstitution:
To prepare stock, a quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL.
 
 
Storage:
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

IL-18 binding protein

IL-18BP is a secreted protein that binds IL-18 with high affinity and prevents its interaction with the IL-18R. Different isoforms of IL-18BP have been demonstrated, four in human and two in the murine system, and biological activity is limited to particular isoforms.IL-18 binding protein (IL-18BP) is a constitutively expressed and secreted protein that resembles the extracellular domains of Ig-like receptors but has no transmembrane form.Because IL-18 is one of the early signals leading to IFN-gamma production by T- helper (Th) type-1 cells, blocking IL-18 activity by IL-18BP may be involved in down-modulation of the early phases of immune responses.

Related Publications:
inflammation in the central nervous system and th17 responses are inhibited by ifn-–induced IL-18 binding protein
J. Immunol., Aug 2010; 185: 2458 - 2466.
IL-18 binding protein-expressing mesenchymal stem cells improve myocardial protection after ischemia or infarction
PNAS, Oct 2009; 106: 17499 - 17504.
multimodality imaging of il-18–binding protein-fc therapy of experimental lung metastasis
Clin. Cancer Res., Oct 2008; 14: 6137 - 6145.
co-expression of IL-18 binding protein and il-4 regulates th1/th2 cytokine response in murine collagen-induced arthritis
Acta Biochim Biophys Sin, Feb 2008; 40: 116 - 124.
targeted overexpression of IL-18 binding protein at the central nervous system overrides flexibility in functional polarization of antigen-specific th2 cells
J. Immunol., Apr 2005; 174: 4307 - 4315.




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