Human Follistatin Recombinant
Categories: CytokinesRecombinant Human Cytokines$160.00 – $4,700.00
Description
Accession
P19883
Source
Optimized DNA sequence encoding Human Follistatin mature chain was expressed inHek293 cells.
Molecular weight
Native human Follistatin, generated by the proteolytic removal of the signal peptide and propeptide,the molecule has a calculated molecular mass of approximately31 kDa. Recombinant Follistatin is a monomer protein consisting of 296 amino acid residue subunits, due to glycosylation migrates as an approximately 45 kDa protein under non-reducingand reducing conditions in SDS-PAGE.
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
The ED(50) was determined by theability to neutralize Activin A inhibitory effect of mouse MPC-11 cells and was determined to be 0.1-0.4 µg/ml in the presence of 7.5 ng/ml Activin A.
Protein Sequence
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human Follistatin was lyophilized at 1mg/ml containing no additives.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
